Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E184] to alpha smooth muscle Actin (HRP)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-alpha smooth muscle Actin antibody [E184] (HRP)
See all alpha smooth muscle Actin primary antibodies
DescriptionRabbit monoclonal [E184] to alpha smooth muscle Actin (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
- WB: A431 whole cell lysate. IHC-P: normal human kidney tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 647) (ab196919)
- Anti-alpha smooth muscle Actin antibody [E184] (PE) (ab209435)
- Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 405) (ab210128)
- Anti-alpha smooth muscle Actin antibody [E184] - BSA and Azide free (ab215368)
- Anti-alpha smooth muscle Actin antibody [E184] (FITC) (ab223920)
- Anti-alpha smooth muscle Actin antibody [E184] (APC) (ab223921)
- Anti-alpha smooth muscle Actin antibody [E184] (ab32575)
Our Abpromise guarantee covers the use of ab196920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
Anti-alpha smooth muscle Actin antibody [E184] (HRP) (ab196920) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 2 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196920 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of alpha smooth muscle Actin staining in a section of formalin-fixed paraffin-embedded normal human kidney tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196920 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab196920 has not yet been referenced specifically in any publications.