Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E184] to alpha smooth muscle Actin (PE)
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Rat
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-alpha smooth muscle Actin antibody [E184] (PE)
See all alpha smooth muscle Actin primary antibodies
DescriptionRabbit monoclonal [E184] to alpha smooth muscle Actin (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide within Human alpha smooth muscle Actin (N terminal). The exact sequence is proprietary.
- Flow Cyt: SV40LT-SMC cells ICC/IF: SV40LT-SMC cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 647) (ab196919)
- Anti-alpha smooth muscle Actin antibody [E184] (HRP) (ab196920)
- Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 488) (ab197240)
- Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 405) (ab210128)
- Anti-alpha smooth muscle Actin antibody [E184] - BSA and Azide free (ab215368)
- Anti-alpha smooth muscle Actin antibody [E184] (FITC) (ab223920)
- Anti-alpha smooth muscle Actin antibody [E184] (APC) (ab223921)
- Anti-alpha smooth muscle Actin antibody [E184] (ab32575)
Our Abpromise guarantee covers the use of ab209435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.
This product gave a positive signal in SV40LT cells fixed with 4% formaldehyde (10 min)
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
Overlay histogram showing SV40LT-SMC cells stained with ab209435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209435, 1/5000 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.
This antibody gave a positive signal in SV40LT-SMC cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab209435 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209435 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab209435 has not yet been referenced specifically in any publications.