Key features and details
- Mouse monoclonal [SPM332] to alpha smooth muscle Actin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-alpha smooth muscle Actin antibody [SPM332]
See all alpha smooth muscle Actin primary antibodies
DescriptionMouse monoclonal [SPM332] to alpha smooth muscle Actin
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Goat, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Baboon
Synthetic peptide within Human alpha smooth muscle Actin conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Database link: P62736
- IHC-P: Human leiomyosarcoma tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate.
Light chain typekappa
sELISA pair antibody
Our Abpromise guarantee covers the use of ab270251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IF||Use a concentration of 1 - 2 µg/ml.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
Immunofluorescence analysis of HeLa cells labeling alpha smooth muscle Actin using ab270251 at 2µg/ml, followed by goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot (Red).
Formalin-fixed, paraffin-embedded human leiomyosarcoma tissue stained for alpha smooth muscle Actin using ab270251 at 2µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270251 has not yet been referenced specifically in any publications.