Key features and details
- Rabbit polyclonal to Alpha SNAP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Alpha SNAP antibody
See all Alpha SNAP primary antibodies
DescriptionRabbit polyclonal to Alpha SNAP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant full length protein corresponding to Human Alpha SNAP aa 1-295.
MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAAN MFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEA INCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSAD YYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKY SAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEA HEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR
Database link: P54920
- WB: HEK-293T, HeLa and K562 whole cell lysates. IHC-P: Human liver cancer tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionRequired for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
Sequence similaritiesBelongs to the SNAP family.
- Information by UniProt
- Alpha soluble NSF attachment protein antibody
- Alpha-soluble NSF attachment protein antibody
- N ethylmaleimide sensitive factor attachment protein alpha antibody
All lanes : Anti-Alpha SNAP antibody (ab237814) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 33 kDa
Paraffin-embedded human liver cancer tissue stained for Alpha SNAP using ab237814 at 1/100 dilution in immunohistochemical analysis.
MCF7 (human breast adenocarcinoma cell line) cells stained for Alpha SNAP (green) using ab237814 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237814 has not yet been referenced specifically in any publications.