Product nameAnti-Alpha SNAP antibody [EPR7372]
See all Alpha SNAP primary antibodies
DescriptionRabbit monoclonal [EPR7372] to Alpha SNAP
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human Alpha SNAP. (P54920)
- HeLa cell lysate, Molt4 cell lysate, HepG2 cells and cell lysate, Human kidney tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol
|ICC/IF||1/100 - 1/250.|
FunctionRequired for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
Sequence similaritiesBelongs to the SNAP family.
- Information by UniProt
- Alpha soluble NSF attachment protein antibody
- Alpha-soluble NSF attachment protein antibody
- N ethylmaleimide sensitive factor attachment protein alpha antibody
All lanes : Anti-Alpha SNAP antibody [EPR7372] (ab133673) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt4 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with ab133673 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with ab133673 at 1/100.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133673 has been referenced in 1 publication.
- Huang X et al. Mechanistic insights into the SNARE complex disassembly. Sci Adv 5:eaau8164 (2019). PubMed: 30989110