Recombinant Anti-Alpha SNAP antibody [EPR7372] (ab133673)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7372] to Alpha SNAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Alpha SNAP antibody [EPR7372]
See all Alpha SNAP primary antibodies -
Description
Rabbit monoclonal [EPR7372] to Alpha SNAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cell lysate, Molt4 cell lysate, HepG2 cells and cell lysate, Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7372 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
ICC/IF
1/100 - 1/250. |
Target
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Function
Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. -
Sequence similarities
Belongs to the SNAP family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8775 Human
- Entrez Gene: 108124 Mouse
- Entrez Gene: 140673 Rat
- Omim: 603215 Human
- SwissProt: P54920 Human
- SwissProt: Q9DB05 Mouse
- SwissProt: P54921 Rat
- Unigene: 126938 Human
see all -
Alternative names
- Alpha soluble NSF attachment protein antibody
- Alpha-soluble NSF attachment protein antibody
- N ethylmaleimide sensitive factor attachment protein alpha antibody
see all
Images
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All lanes : Anti-Alpha SNAP antibody [EPR7372] (ab133673) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt4 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with ab133673 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with ab133673 at 1/100.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab133673 has been referenced in 2 publications.
- Huang X et al. Mechanistic insights into the SNARE complex disassembly. Sci Adv 5:eaau8164 (2019). PubMed: 30989110
- Lin Y et al. SNARE-Mediated Cholesterol Movement to Mitochondria Supports Steroidogenesis in Rodent Cells. Mol Endocrinol 30:234-47 (2016). PubMed: 26771535