Recombinant
RabMAb

Recombinant Anti-Alpha SNAP antibody [EPR7372] (ab133673)

Submit a review Submit a question References (1)

Overview

  • Product name

    Anti-Alpha SNAP antibody [EPR7372]
    See all Alpha SNAP primary antibodies

  • Description

    Rabbit monoclonal [EPR7372] to Alpha SNAP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to a region within Human Alpha SNAP. (P54920)

  • Positive control

    • HeLa cell lysate, Molt4 cell lysate, HepG2 cells and cell lysate, Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    Images

    • Western blot - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      Western blot - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      All lanes : Anti-Alpha SNAP antibody [EPR7372] (ab133673) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Molt4 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with ab133673 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with ab133673 at 1/100.
    • Other - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      Other - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    Publishing research using ab133673? Please let us know so that we can cite the reference in this datasheet.

    ab133673 has been referenced in 1 publication.

    • Huang X  et al. Mechanistic insights into the SNARE complex disassembly. Sci Adv 5:eaau8164 (2019). PubMed: 30989110

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab133673.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com