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RecombinantRabMAb

Recombinant Anti-Alpha SNAP antibody [EPR7372] (ab133673)

Submit a review Submit a question References (2)
Western blot - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
  • OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
  • Anti-Alpha SNAP antibody [EPR7372] (ab133673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7372] to Alpha SNAP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Alpha SNAP antibody [EPR7372]
    See all Alpha SNAP primary antibodies

  • Description

    Rabbit monoclonal [EPR7372] to Alpha SNAP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cell lysate, Molt4 cell lysate, HepG2 cells and cell lysate, Human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

Images

  • Western blot - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    Western blot - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    All lanes : Anti-Alpha SNAP antibody [EPR7372] (ab133673) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Molt4 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with ab133673 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with ab133673 at 1/100.
  • OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Alpha SNAP antibody [EPR7372] (ab133673)
    Anti-Alpha SNAP antibody [EPR7372] (ab133673)

References (2)

Publishing research using ab133673? Please let us know so that we can cite the reference in this datasheet.

ab133673 has been referenced in 2 publications.

  • Huang X  et al. Mechanistic insights into the SNARE complex disassembly. Sci Adv 5:eaau8164 (2019). PubMed: 30989110
  • Lin Y  et al. SNARE-Mediated Cholesterol Movement to Mitochondria Supports Steroidogenesis in Rodent Cells. Mol Endocrinol 30:234-47 (2016). PubMed: 26771535

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