Recombinant Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
![Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)](https://www.abcam.com/ps/products/280/ab280382/Images/ab280382-456804-anti-alphasynuclein-antibody-42asynuclein-western-blot-mouse-cerebellum-lysate10-ug.jpg "Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [42/a-Synuclein] to Alpha-synuclein - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free
See all Alpha-synuclein primary antibodies -
Description
Mouse monoclonal [42/a-Synuclein] to Alpha-synuclein - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: Mouse cerebellum, Human cerebellum, Rat hippocampus. IHC-P: Rat cerebrum, Mouse cerebrum, Human cerebrum.
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General notes
ab280382 is the carrier-free version of ab280377.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
42/a-Synuclein -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
Tissue specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
Involvement in disease
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similarities
Belongs to the synuclein family. -
Domain
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localization
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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Database links
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 21374 Human
see all -
Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
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Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)All lanes : Anti-Alpha-synuclein antibody [42/a-Synuclein] (ab280377) at 1/1000 dilution
Lane 1 : Mouse cerebellum lysate at 20 µg
Lane 2 : Mouse kidney lysate at 20 µg
Lane 3 : Human cerebellum lysate at 20 µg
Lane 4 : Human kidney lysate at 20 µg
Lane 5 : Rat hippocampus lysate at 20 µg
Lane 6 : Rat kidney lysate at 10 µg
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab280377, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse kidney, human kidney, rat kidney (PMID:14997013)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:14997013, 30553024).
Exposure time 15 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)](https://www.abcam.com/ps/products/280/ab280382/Images/ab280382-3-anti-alphasynuclein-antibody-42asynuclein-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)This data was developed using ab280377 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in human cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)](https://www.abcam.com/ps/products/280/ab280382/Images/ab280382-2-anti-alphasynuclein-antibody-42asynuclein-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)This data was developed using ab280377 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in mouse cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)](https://www.abcam.com/ps/products/280/ab280382/Images/ab280382-1-anti-alphasynuclein-antibody-42asynuclein-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)This data was developed using ab280377 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Alpha-synuclein with abab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in rat cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)](https://www.abcam.com/ps/products/280/ab280382/Images/ab280382-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (ab280382)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab280382 has not yet been referenced specifically in any publications.
