- Datasheet
- Specific References (28)
- Protocols
Overview
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Product nameAnti-Alpha-synuclein antibody [4D6]
See all Alpha-synuclein primary antibodies -
DescriptionMouse monoclonal [4D6] to Alpha-synuclein
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Host speciesMouse
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Tested applicationsSuitable for: IHC-FoFr, WB, IHC-P, IHC-Fr, ELISAmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant full length protein corresponding to Human Alpha-synuclein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesAmmonium sulfate precipitated and dialyzed tissue culture supernatant.
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ClonalityMonoclonal
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Clone number4D6
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab1903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/300. PubMed: 17227870Perfuse with 4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer and postfix. | |
| WB | 1/100 - 1/10000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. | |
| IHC-Fr | 1/100 - 1/1000. | |
| ELISA | Use at an assay dependent concentration. |
Application notes
Is unsuitable for Flow Cyt or ICC.
Target
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FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
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Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
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Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similaritiesBelongs to the synuclein family.
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DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
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Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
- Information by UniProt
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Database links
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 21374 Human
see all -
Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
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![Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)](https://www.abcam.com/ps/products/1/ab1903/Images/alpha-Synuclein-Primary-antibodies-ab1903-2.jpg)
Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)All lanes : Anti-Alpha-synuclein antibody [4D6] (ab1903) at 5 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Additional bands at: 18 kDa (possible post-translational modification), 80 kDa (possible post-translational modification) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)](https://www.abcam.com/ps/products/1/ab1903/Images/ab1903_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)IHC-P using ab1903 showing lewy bodies in a substantia nigra neuron(x400)
References
This product has been referenced in:
- Gerson JE et al. Tau oligomers mediate a-synuclein toxicity and can be targeted by immunotherapy. Mol Neurodegener 13:13 (2018). Read more (PubMed: 29544548) »
- Wang J et al. Protein arginylation targets alpha synuclein, facilitates normal brain health, and prevents neurodegeneration. Sci Rep 7:11323 (2017). WB . Read more (PubMed: 28900170) »
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