Anti-Alpha-synuclein antibody [4D6] (ab1903)

Reviews (4)Q&A (4)References (41)

Overview

  • Product name

    Anti-Alpha-synuclein antibody [4D6]
    See all Alpha-synuclein primary antibodies

  • Description

    Mouse monoclonal [4D6] to Alpha-synuclein

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-FoFr, WB, IHC-P, IHC-Fr, ELISAmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein corresponding to Human Alpha-synuclein.

Properties

Applications

Our Abpromise guarantee covers the use of ab1903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300. PubMed: 17227870Perfuse with 4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer and postfix.
WB 1/100 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IHC-Fr 1/100 - 1/1000.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

    • Tissue specificity

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

    • Involvement in disease

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body

    • Sequence similarities

      Belongs to the synuclein family.

    • Domain

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.

    • Post-translational
      modifications

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.

    • Cellular localization

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • SNCA antibody
      • Snca synuclein antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
      Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)This image is taken from Migdalska-Richards A. et al. Ann Neurol. 2016 Nov; 80(5): 766–775. Fig 5 doi: 10.1002/ana.24790

      α-Synuclein (α-syn) and S129-phosphorylated α-synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α-synuclein (using ab1903) and serine 129 (S129)-phosphorylated α-synuclein protein (using ab51253) in the brainstem (example blots shown).

    • Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
      Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
      All lanes : Anti-Alpha-synuclein antibody [4D6] (ab1903) at 5 µg/ml

      Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate

      Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 16 kDa
      Observed band size: 16 kDa
      Additional bands at: 18 kDa (possible post-translational modification), 80 kDa (possible post-translational modification)



    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)

      IHC-P using ab1903 showing lewy bodies in a human substantia nigra neuron(x400)

    References

    This product has been referenced in:

    • Lothian A  et al. Characterization of the metal status of natively purified alpha-synuclein from human blood, brain tissue, or recombinant sources using size exclusion ICP-MS reveals no significant binding of Cu, Fe or Zn. Metallomics 11:128-140 (2019). Read more (PubMed: 30465671) »
    • Laurencé C  et al. A new human pyridinium metabolite of furosemide, inhibitor of mitochondrial complex I, is a candidate inducer of neurodegeneration. Biochem Pharmacol 160:14-23 (2019). Read more (PubMed: 30537467) »
    See all 41 Publications for this product

    Publishing research using ab1903? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Question

    I was wondering whether it is possible to ask for the peptides for immunization to produce this antibody since I see from a different question that the epitopes have not been mapped?

    Read More
    Answer

    I would like to confirm that the epitope for this antibody has not been mapped and that the immunogen used was not a peptide but the full length protein.

    The alternative antibodies we have against this target for which the epitopes have been mapped are:

    > ab138501: rabbit monoclonal antibody, KO validated, that binds to amino acids 118-123 (VDPDNE). https://www.abcam.com/ab138501.

    > ab27766: mouse monoclonal antibody that binds in the region encoded by amino acids 115-122 of alpha-synuclein. https://www.abcam.com/ab27766.

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Synuclein antibody [4D6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (cerebral cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 20 min, 100C
    Permeabilization
    No
    Specification
    cerebral cortex
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 16 2016

    Western blot abreview for Anti-alpha Synuclein antibody [4D6]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Tissue lysate - whole (CNS tissue)
    Specification
    CNS tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 01 2014

    Western blot abreview for Anti-alpha Synuclein antibody [4D6]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (Brain Tissue)
    Specification
    Brain Tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2014

    Western blot abreview for Anti-alpha Synuclein antibody [4D6]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Loading amount
    10 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Question

    Good afternoon Cold you please tell me if ab1903 (mouse monoclonal antibody to alpha Synuclein) recognises either non-phosphorylated or phosphorylated alpha synuclein, or both.

    Read More
    Answer

    Thank you for your email. We have not checked for specificity against the phosphorylated and non-phosphorylated form of alpha synuclein, therefore we do not have this information. If you do decide to test this antibody for specificity we would be happy to hear your feedback. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

    Read More

    Question

    What are the epitopes recognized by each of these alpha synuclein antibodies?

    Read More
    Answer

    The epitopes that are recognized by the synuclein antibodies ab1904, ab1903, ab24717, and ab36615 have not been mapped. I am sorry we could not be of more help. If you have any other questions, please do not hesitate to contact us.

    Read More

    Question

    Customer is interested in using this antibody in IHC with mouse tissue; should a Mouse on Mouse kit be used?

    Read More
    Answer

    Thank you for your enquiry. We guarantee that this antibody will work in IHC and cross-react with mouse alpha synuclein. We would recommend using this antibody as per our general IHC protocol (protocols for both frozen and formalin-fixed paraffin-embedded sections can be found by clicking on the protocols tab located on the online datasheet). However, if after you experience problems with high background, using a Mouse on Mouse kit from Vector would be suggested. Please contact us again if you have any additional questions.

    Read More

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