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    alpha-synuclein-antibody-4d6-ab1903.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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Anti-Alpha-synuclein antibody [4D6] (ab1903)

  • Datasheet
Reviews (4)Q&A (4)References (48)

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Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
  • Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)

Key features and details

  • Mouse monoclonal [4D6] to Alpha-synuclein
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Peptide
Alpha-synuclein (phospho S129) peptide (ab188826)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
Product image
Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

View more associated products

Overview

  • Product name

    Anti-Alpha-synuclein antibody [4D6]
    See all Alpha-synuclein primary antibodies
  • Description

    Mouse monoclonal [4D6] to Alpha-synuclein
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Alpha-synuclein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Ammonium sulfate precipitated and dialyzed tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4D6
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Synuclein
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)
    • Recombinant Human Alpha-synuclein (mutated A53T) protein (ab51184)
    • Recombinant Human Alpha-synuclein protein (ab51189)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (3)
1/100 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P (1)
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
Notes
WB
1/100 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificity

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in disease

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similarities

      Belongs to the synuclein family.
    • Domain

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational
      modifications

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localization

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Target information above from: UniProt accession P37840 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6622 Human
      • Omim: 163890 Human
      • SwissProt: P37840 Human
      • Unigene: 21374 Human
      • Alternative names

        • Alpha synuclein antibody
        • Alpha-synuclein antibody
        • Alpha-synuclein, isoform NACP140 antibody
        • alphaSYN antibody
        • MGC105443 antibody
        • MGC110988 antibody
        • MGC127560 antibody
        • MGC64356 antibody
        • NACP antibody
        • Non A beta component of AD amyloid antibody
        • Non A4 component of amyloid antibody
        • Non A4 component of amyloid precursor antibody
        • Non-A beta component of AD amyloid antibody
        • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
        • Non-A4 component of amyloid precursor antibody
        • Non-A4 component of amyloid, precursor of antibody
        • OTTHUMP00000218549 antibody
        • OTTHUMP00000218551 antibody
        • OTTHUMP00000218552 antibody
        • OTTHUMP00000218553 antibody
        • OTTHUMP00000218554 antibody
        • PARK 1 antibody
        • PARK 4 antibody
        • PARK1 antibody
        • PARK4 antibody
        • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
        • Parkinson disease familial 1 antibody
        • SNCA antibody
        • Snca synuclein antibody
        • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYN antibody
        • Synuclein alpha antibody
        • Synuclein alpha 140 antibody
        • Synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYUA_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
        Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)This image is taken from Migdalska-Richards A. et al. Ann Neurol. 2016 Nov; 80(5): 766–775. Fig 5 doi: 10.1002/ana.24790

        α-Synuclein (α-syn) and S129-phosphorylated α-synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α-synuclein (using ab1903) and serine 129 (S129)-phosphorylated α-synuclein protein (using ab51253) in the brainstem (example blots shown).

      • Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
        Western blot - Anti-Alpha-synuclein antibody [4D6] (ab1903)
        All lanes : Anti-Alpha-synuclein antibody [4D6] (ab1903) at 5 µg/ml

        Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
        Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate

        Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 16 kDa
        Observed band size: 16 kDa
        Additional bands at: 18 kDa (possible post-translational modification), 80 kDa (possible post-translational modification)



      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] (ab1903)

        IHC-P using ab1903 showing lewy bodies in a human substantia nigra neuron(x400)

      Protocols

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (48)

      Publishing research using ab1903? Please let us know so that we can cite the reference in this datasheet.

      ab1903 has been referenced in 48 publications.

      • Ho PW  et al. Age-dependent accumulation of oligomeric SNCA/a-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Autophagy 16:347-370 (2020). PubMed: 30983487
      • Schiffer V  et al. Characterization of the visceral and neuronal phenotype of 4L/PS-NA mice modeling Gaucher disease. PLoS One 15:e0227077 (2020). PubMed: 31929594
      • Wu J  et al. The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson's Disease. Oxid Med Cell Longev 2020:8735249 (2020). PubMed: 32724496
      • Yang SY  et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
      • Brozzetti L  et al. Neurodegeneration-Associated Proteins in Human Olfactory Neurons Collected by Nasal Brushing. Front Neurosci 14:145 (2020). PubMed: 32194369
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-8 of 8 Abreviews or Q&A

      Question

      I was wondering whether it is possible to ask for the peptides for immunization to produce this antibody since I see from a different question that the epitopes have not been mapped?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 29 2017

      Answer

      I would like to confirm that the epitope for this antibody has not been mapped and that the immunogen used was not a peptide but the full length protein.

      The alternative antibodies we have against this target for which the epitopes have been mapped are:

      > ab138501: rabbit monoclonal antibody, KO validated, that binds to amino acids 118-123 (VDPDNE). https://www.abcam.com/ab138501.

      > ab27766: mouse monoclonal antibody that binds in the region encoded by amino acids 115-122 of alpha-synuclein. https://www.abcam.com/ab27766.

      Read More

      Abcam Scientific Support

      Answered on Sep 29 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Synuclein antibody [4D6]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (cerebral cortex)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 20 min, 100C
      Permeabilization
      No
      Specification
      cerebral cortex
      Blocking step
      H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 16 2016

      Western blot abreview for Anti-alpha Synuclein antibody [4D6]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing (12)
      Sample
      Human Tissue lysate - whole (CNS tissue)
      Specification
      CNS tissue
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 01 2014

      Western blot abreview for Anti-alpha Synuclein antibody [4D6]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      75 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (Brain Tissue)
      Specification
      Brain Tissue
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 13 2014

      Western blot abreview for Anti-alpha Synuclein antibody [4D6]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain)
      Loading amount
      10 µg
      Specification
      Brain
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 16 2013

      Question

      Good afternoon Cold you please tell me if ab1903 (mouse monoclonal antibody to alpha Synuclein) recognises either non-phosphorylated or phosphorylated alpha synuclein, or both.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 07 2011

      Answer

      Thank you for your email. We have not checked for specificity against the phosphorylated and non-phosphorylated form of alpha synuclein, therefore we do not have this information. If you do decide to test this antibody for specificity we would be happy to hear your feedback. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

      Read More

      Abcam Scientific Support

      Answered on Sep 07 2011

      Question

      What are the epitopes recognized by each of these alpha synuclein antibodies?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 14 2007

      Answer

      The epitopes that are recognized by the synuclein antibodies ab1904, ab1903, ab24717, and ab36615 have not been mapped. I am sorry we could not be of more help. If you have any other questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Feb 14 2007

      Question

      Customer is interested in using this antibody in IHC with mouse tissue; should a Mouse on Mouse kit be used?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 12 2006

      Answer

      Thank you for your enquiry. We guarantee that this antibody will work in IHC and cross-react with mouse alpha synuclein. We would recommend using this antibody as per our general IHC protocol (protocols for both frozen and formalin-fixed paraffin-embedded sections can be found by clicking on the protocols tab located on the online datasheet). However, if after you experience problems with high background, using a Mouse on Mouse kit from Vector would be suggested. Please contact us again if you have any additional questions.

      Read More

      Abcam Scientific Support

      Answered on Jan 12 2006

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