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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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Anti-Alpha-synuclein antibody (ab21976)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (ab21976)

    Key features and details

    • Sheep polyclonal to Alpha-synuclein
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Alpha-synuclein antibody
      See all Alpha-synuclein primary antibodies
    • Description

      Sheep polyclonal to Alpha-synuclein
    • Host species

      Sheep
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human Alpha-synuclein.
      Sequence:

      PQEGILEDMPVDP

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes


      This antibody achieves excellent staining of Lewy bodies in Parkinson’s disease and Dementia with Lewy bodies disease tissue and gives excellent staining of glial inclusions in Multi System Atrophy tissue.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Purification notes

      This product is supplied as filtered serum.
    • Primary antibody notes

      This antibody achieves excellent staining of Lewy bodies in Parkinson’s disease and Dementia with Lewy bodies disease tissue and gives excellent staining of glial inclusions in Multi System Atrophy tissue.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Synuclein
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau

    Associated products

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Recombinant Protein

      • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab21976 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    1/1000 - 1/4000. Antigen retrieval has been used in characterisation but may not be necessary. 1) Slide holder containing 1mM EDTA at pH 8 is boiled in a microwave (~2min). 2) Slides are placed in holder and boiled for a further 10 min with the microwave on low. 3) Slides allowed to cool before use.
    Notes
    IHC-P
    1/1000 - 1/4000. Antigen retrieval has been used in characterisation but may not be necessary. 1) Slide holder containing 1mM EDTA at pH 8 is boiled in a microwave (~2min). 2) Slides are placed in holder and boiled for a further 10 min with the microwave on low. 3) Slides allowed to cool before use.

    Target

    • Function

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificity

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in disease

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similarities

      Belongs to the synuclein family.
    • Domain

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational
      modifications

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localization

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Target information above from: UniProt accession P37840 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6622 Human
      • Omim: 163890 Human
      • SwissProt: P37840 Human
      • Unigene: 21374 Human
      • Alternative names

        • Alpha synuclein antibody
        • Alpha-synuclein antibody
        • Alpha-synuclein, isoform NACP140 antibody
        • alphaSYN antibody
        • MGC105443 antibody
        • MGC110988 antibody
        • MGC127560 antibody
        • MGC64356 antibody
        • NACP antibody
        • Non A beta component of AD amyloid antibody
        • Non A4 component of amyloid antibody
        • Non A4 component of amyloid precursor antibody
        • Non-A beta component of AD amyloid antibody
        • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
        • Non-A4 component of amyloid precursor antibody
        • Non-A4 component of amyloid, precursor of antibody
        • OTTHUMP00000218549 antibody
        • OTTHUMP00000218551 antibody
        • OTTHUMP00000218552 antibody
        • OTTHUMP00000218553 antibody
        • OTTHUMP00000218554 antibody
        • PARK 1 antibody
        • PARK 4 antibody
        • PARK1 antibody
        • PARK4 antibody
        • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
        • Parkinson disease familial 1 antibody
        • SNCA antibody
        • Snca synuclein antibody
        • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYN antibody
        • Synuclein alpha antibody
        • Synuclein alpha 140 antibody
        • Synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYUA_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (ab21976)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (ab21976)
        ab21976 staining Lewy body in Dementia with Lewy body disease tissue at 1/1000 dilution by Immunohistochemistry (FFPE-sections). Secondary antibody biotinylated Donkey and sheep followed by Vector ABC and DAB substrate.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab21976? Please let us know so that we can cite the reference in this datasheet.

      ab21976 has been referenced in 2 publications.

      • McGlinchey RP  et al. C-terminal a-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem 294:9973-9984 (2019). PubMed: 31092553
      • Yap TL  et al. Alpha-synuclein interacts with Glucocerebrosidase providing a molecular link between Parkinson and Gaucher diseases. J Biol Chem 286:28080-8 (2011). PubMed: 21653695

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Synuclein antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (iPS cell derived neurons)
      Permeabilization
      Yes - see blocking buffer
      Specification
      iPS cell derived neurons
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 25 2016

      Western blot abreview for Anti-alpha Synuclein antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing (10% SDS gel)
      Sample
      Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))
      Specification
      human iPS cell derived neurons (healthy/diseased)
      Treatment
      quinolinic acid 72h (and non treated) 500nM
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 19 2014

      Question

      Many thanks for your help with the order of sheep polyclonal to alpha-synuclein antibody (ab21976). I checked the data sheet and found the immunohistochemistry image of this antibody (Formalin-fixed paraffin-embedded sections), but didn’t find the information for the pre-treatment. Can you kindly provide me the proper pre-treatment for this Ab (ab21976)?  Thanks again, any queries, please contact me.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 12 2011

      Answer

      Thank you for your inquiry. This antibody has been characterized using antigen retreival in the following way. 1) Slide holder containing 1mM EDTA at pH 8 is boiled in a microwave (~2min). 2) Slides are placed in holder and boiled for a further 10 min with the microwave on low. 3) Slides allowed to cool before use. It is important that your tissue is placed on high binding glass slides so the tissue does not come off. Lomb (Menzel-glaser) Superfrost Plus slides were used with this protocol. I hope this information helps. Please contact us with any other questions.

      Read More

      Abcam Scientific Support

      Answered on Aug 12 2011

      Question

      Dear Abcam: AS the Specificity of ab21976, you say, "The antibody to human beta synuclein antibody (aa 108-125)?(aa 108-120) is specific and does not cross react with either alpha or gamma synuclein." I assume this is the specificity of one of your beta synuclein antibodies. Would you replace this with the real specificity of 21976? Thank you,

      Read More

      Abcam community

      Verified customer

      Asked on May 29 2006

      Answer

      Thank you for your enquiry and for bringing this error to our attention. I have corrected the online datasheet, changed the immunogen information to state that the immunogen is amino acids 108-120 of alpha synuclein, and changed the specificity to state that the antibody does not cross-react with beta or gamma synuclein. I apologize for the confusion caused by the previous datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jun 01 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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