• Product name

    Anti-Alpha-synuclein antibody
    See all Alpha-synuclein primary antibodies
  • Description

    Sheep polyclonal to Alpha-synuclein
  • Host species

  • Specificity

    Human and rat alpha synuclein. Cross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not yet been tested.
  • Tested applications

    Suitable for: IHC-FoFr, Dot blot, IHC-Fr, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Alpha-synuclein aa 116-131. Synthetic peptide of human synuclein (116-131) coupled to diphtheria toxoid with cysteine residue attached to N-ter of peptide. Conjugate cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).




Our Abpromise guarantee covers the use of ab6162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use a concentration of 2 µg/ml.
Dot blot Use a concentration of 0.5 µg/ml.
IHC-Fr 1/500 - 1/1000.
IHC-P Use a concentration of 1 µg/ml. PubMed: 20106867
WB Use a concentration of 0.5 µg/ml.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificity

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in disease

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similarities

      Belongs to the synuclein family.
    • Domain

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localization

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • SNCA antibody
      • Snca synuclein antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all


    • ab6162 at a 1/40 dilution staining human brain tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with serum before incubation with ab6162 for 1 hour. A vector ABC kit was used to detect bound antibody.

      See Abreview

    • IHC-FoFr image of Alpha Synuclein staining on alpha Synuclein Trasgenic mouse brain section stained using ab6162. The animal were perfuse with 4% PFA. The sections were incubated in 10% normal donkey serum in 0.1% PBS- and triton 0.3X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody ab 6162, 2 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 anti-sheep IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      See Abreview

    • All lanes : Anti-Alpha-synuclein antibody (ab6162) at 1 µg/ml

      Lane 1 : Recombinant Human a -synuclein at 0.1 µg
      Lane 2 : Mouse brain lysate at 30 µg

      All lanes : anti-sheep HRP at 1/6000 dilution
    • ab6162 staining Alpha-synuclein in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with PB ab64226 for 10 minutes at room temperature and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200) for 30 minutes. An undiluted HRP-conjugated rabbit anti-goat polyclonal was used as the secondary antibody.

      See Abreview


    This product has been referenced in:

    See all 22 Publications for this product

    Customer reviews and Q&As

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jan 23 2018

    This product is known to not work in this application or species.
    Immunocytochemistry/ Immunofluorescence
    Rat Cell (synaptosomes isolated from rat brain)
    Yes - 0.05 % Tween 20
    synaptosomes isolated from rat brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 24 2015


    Thank you for calling Abcam yesterday.

    I have heard back from the lab about the buffer of ab6162. All of our affinity purified antibody ultimately end up in PBS from which they are then lyophilized, thus all that is remaining are some buffer salts and antibody protein once dried. Initial composition was 50mM Na+Phosphate, 150mM NaCL, final pH 7.5. There are no preservatives.

    I hope this helps, please let me know if there is anything else I can help you with.

    Read More


    Thank you very much for your enquiry.

    Unfortunately, we currently do not have a particular ready-to-useIHC Kit available that has been optimised for the use of a sheep polyclonal primary antibody with rat tissue.

    Therefore, if you do not have the appropriate components available,I may recommend the individual components for IHC which are listed in more detail at https://www.abcam.com/index.html?pageconfig=resource&rid=12853.

    For example, I would suggest one of the secondary antibodiesab6747 or ab97125:
    https://www.abcam.com/index.html?datasheet=6747 (or use the following: https://www.abcam.com/index.html?datasheet=6747).
    https://www.abcam.com/index.html?datasheet=97125 (or use the following: https://www.abcam.com/index.html?datasheet=97125).

    As you may see, we also have
    buffers (https://www.abcam.com/index.html?pageconfig=resource&rid=12854),
    diluents (https://www.abcam.com/index.html?pageconfig=resource&rid=12864),
    blocking solutions/serum (https://www.abcam.com/index.html?pageconfig=resource&rid=12859or https://www.abcam.com/index.html?pageconfig=resource&rid=12889)
    and substrates (https://www.abcam.com/index.html?pageconfig=resource&rid=12908or https://www.abcam.com/index.html?pageconfig=resource&rid=12886)
    for you to tailor your specific experiments with rat brain sectionsavailable.

    These include for example the following products:

    ab64214: https://www.abcam.com/index.html?datasheet=64214 (or use the following: https://www.abcam.com/index.html?datasheet=64214).

    ab64216: https://www.abcam.com/index.html?datasheet=64216 (or use the following: https://www.abcam.com/index.html?datasheet=64216).

    ab64211: https://www.abcam.com/index.html?datasheet=64211 (or use the following: https://www.abcam.com/index.html?datasheet=64211).

    ab64226: https://www.abcam.com/index.html?datasheet=64226 (or use the following: https://www.abcam.com/index.html?datasheet=64226).

    ab64238: https://www.abcam.com/index.html?datasheet=64238 (or use the following: https://www.abcam.com/index.html?datasheet=64238).

    Also, I have attached our protocols for your consideration.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More


    The production and formulation of this sheep antibody to alpha synuclein has not changed since 2009. I hope this is helpful. Please contact us again if you have any further questions.

    Read More
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Mouse Tissue sections (Alpha Synuclein Transgenic Mouse brain)
    Alpha Synuclein Transgenic Mouse brain
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 18 2011

    Immunohistochemistry (Frozen sections)
    Human Tissue sections (Brain)
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: Vector ABC

    Abcam user community

    Verified customer

    Submitted Jul 21 2006


    We assume you are staining for Lewy bodies in human diseased brains. We are using this antibody every week. The staining of Lewy bodies is really quite superb, either in paraffin or cryostat sections. The human tissue is fixed in Zamboni's fixative (4% formaldehyde with 15% saturated picric acid) and used without antigen retrieval. Occassionally we have found some minor improvement using antigen retrieval in 0.1M citrate buffer pH 6.0 improves staining but we rarely use it as staining is always clear.

    Read More


    This antibody works very well on paraffin sections. The antibody will need to be used at a concentration a little higher than for cryostat sections, about 1/500 to 1/1000.

    Read More

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