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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

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  • SDS
Reviews (6)Q&A (2)References (65)

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Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunocytochemistry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunoprecipitation - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunoprecipitation - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
  • Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJFR1] to Alpha-synuclein
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ELISA, ICC/IF
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Primary
Product image
Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Protein
Product image
Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)
Knockout
Product image
Human SNCA (Alpha-synuclein) knockout HEK-293T cell line (ab255433)

View more associated products

Overview

  • Product name

    Anti-Alpha-synuclein antibody [MJFR1]
    See all Alpha-synuclein primary antibodies
  • Description

    Rabbit monoclonal [MJFR1] to Alpha-synuclein
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein within Human Alpha-synuclein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P37840

  • Epitope

    The epitope was mapped to amino acids 118-123 (VDPDNE).
  • Positive control

    • WB: Recombinant Human Alpha-synuclein protein (ab51189), HAP1 and HEK293-T cell lysate; Human brain whole cell lysate; Human brain tissue lysate. IHC-P: FFPE Human Normal Cerebral Cortex; FFPE Human Normal and Parkinson Substantia Nigra tissue. Flow Cyt: Hap1 cells. ICC: Hap1 and U87-MG cells.
  • General notes

    Alpha-Synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.

    This antibody was developed with support from The Michael J. Fox Foundation, in collaboration with the laboratory of Dr. Michael Schlossmacher (University of Ottawa).

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MJFR1
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Synuclein
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Alpha-synuclein antibody [MJFR1] (ab195025)
    • PE Anti-Alpha-synuclein antibody [MJFR1] (ab209306)
    • Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (ab209420)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human SNCA (Alpha-synuclein) knockout HEK-293T cell line (ab255433)
  • KO cell lysates

    • Human SNCA (Alpha-synuclein) knockout HEK-293T cell lysate (ab263769)
  • KO cell pellets

    • Human SNCA (Alpha-synuclein) knockout HEK293T cell pellet (ab278930)
  • Positive Controls

    • Recombinant Human Alpha-synuclein protein (ab51189)
  • Recombinant Protein

    • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)
    • Recombinant Human Alpha-synuclein (mutated A53T) protein (ab51184)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab138501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
WB (4)
1/10000. Predicted molecular weight: 14 kDa.

For unpurified use at  1/1000.00000 - 1/10000.00000

IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/15 to 1/300.

See IHC antigen retrieval protocols.

Flow Cyt
1/200.

For unpurified use at 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/600.

For unpurified use at 1/50

ELISA
Use a concentration of 1 - 10 µg/ml.
ICC/IF (1)
1/150.

For unpurified use at 1/15

Notes
WB
1/10000. Predicted molecular weight: 14 kDa.

For unpurified use at  1/1000.00000 - 1/10000.00000

IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/15 to 1/300.

See IHC antigen retrieval protocols.

Flow Cyt
1/200.

For unpurified use at 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/600.

For unpurified use at 1/50

ELISA
Use a concentration of 1 - 10 µg/ml.
ICC/IF
1/150.

For unpurified use at 1/15

Target

  • Function

    May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
  • Tissue specificity

    Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
  • Involvement in disease

    Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
    Parkinson disease 1
    Parkinson disease 4
    Dementia Lewy body
  • Sequence similarities

    Belongs to the synuclein family.
  • Domain

    The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
  • Post-translational
    modifications

    Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
    Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
    Ubiquitinated. The predominant conjugate is the diubiquitinated form.
    Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
  • Cellular localization

    Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
  • Target information above from: UniProt accession P37840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6622 Human
    • Omim: 163890 Human
    • SwissProt: P37840 Human
    • Unigene: 21374 Human
    • Alternative names

      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • SNCA antibody
      • Snca synuclein antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      All lanes : Anti-Alpha-synuclein antibody [MJFR1] (ab138501) at 1/10000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : SNCA knockout HEK-293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 14 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?



      Lanes 1- 2: Merged signal (red and green). Green - ab138501 observed at 18 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab138501 was shown to react with SNCA in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255433 (knockout cell lysate ab263769) was used. Wild-type HEK-293T and SNCA knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab138501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      IHC image of alpha Synuclein staining in normal Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunocytochemistry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      ab138501 staining Alpha-Synuclein in wild-type Hap1 cells (top panel) and SNCA knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab138501 at 1/10 000 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
    • Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Immunocytochemistry/Immunofluorescence analysis of U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling alpha Synuclein with purified ab138501 at 1/150 dilution (left panel). Cells were fixed with 4% paraformaldehyde. A Goat anti rabbit IgG(Alexa Fluor®555) (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain (right panel).

    • Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      All lanes : Anti-Alpha-synuclein antibody [MJFR1] (ab138501) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : SNCA knockout HAP1 whole cell lysate
      Lane 3 : Human brain whole cell lysate
      Lane 4 : Mouse brain whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 14 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab138501 observed at 14 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab138501 was shown to specifically react with SNCA in wild-type HAP1 cells. No band was observed when SNCA knockout samples were used. Wild-type and SNCA knockout samples were subjected to SDS-PAGE.  Ab138501 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Overlay histogram showing HAP1 wildtype (green line) and HAP1-SNCA knockout cells (red line) stained with ab138501. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab138501, 1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) presorbed (ab150081) at 1/2000 dilution for 30 min at 22°C.

      A rabbit IgG isotype control antibody  (ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-SNCA  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    • Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Western blot - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Anti-Alpha-synuclein antibody [MJFR1] (ab138501) at 1/10000 dilution (purified) + Human fetal brain at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Flow cytometry analysis of 2% paraformaldehyde fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling alpha Synuclein with purified ab138501 at 1/200 dilution. The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution.

      The Isotype control is Rabbit monoclonal IgG (green line).

    • Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Immunocytochemistry/Immunofluorescence analysis of U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling alpha Synuclein with unpurified ab138501 at 1/15 dilution (left panel). Cells were fixed with 4% paraformaldehyde. A Goat anti rabbit IgG(Alexa Fluor®555) (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain (right panel).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      IHC image of alpha Synuclein staining in Normal human Substantia Nigra formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      IHC image of alpha Synuclein staining in Parkinson Human Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunoprecipitation - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunoprecipitation - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      alpha Synuclein was immunoprecipitated from Human fetal brain tissue using purified ab138501 at 1/600 dilution. Western blot was performed from the immunoprecipitate using purified ab138501. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human clear cell carcinoma of kidney labeling alpha Synuclein with purified ab138501 at 1/150 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.

    • Immunoprecipitation - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunoprecipitation - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      alpha Synuclein was immunoprecipitated from Human fetal brain tissue using unpurified ab138501 at 1/50 dilution. Western blot was performed from the immunoprecipitate using unpurified ab138501. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human clear cell carcinoma of kidney labeling alpha Synuclein with unpurified ab138501 at 1/15 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain tissue labeling alpha Synuclein with unpurified ab138501 at 1/300 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Flow Cytometry - Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

      Flow cytometry analysis of 2% paraformaldehyde fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling alpha Synuclein with unpurified ab138501 at 1/20 dilution. The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution.

      The Isotype control is Rabbit monoclonal IgG (green line).

    • Anti-Alpha-synuclein antibody [MJFR1] (ab138501)
      Anti-Alpha-synuclein antibody [MJFR1] (ab138501)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (65)

    Publishing research using ab138501? Please let us know so that we can cite the reference in this datasheet.

    ab138501 has been referenced in 65 publications.

    • He Z  et al. Transmission of tauopathy strains is independent of their isoform composition. Nat Commun 11:7 (2020). PubMed: 31911587
    • Ye Y  et al. N-Terminal Ubiquitination of Amyloidogenic Proteins Triggers Removal of Their Oligomers by the Proteasome Holoenzyme. J Mol Biol 432:585-596 (2020). PubMed: 31518613
    • Ugalde CL  et al. Misfolded a-synuclein causes hyperactive respiration without functional deficit in live neuroblastoma cells. Dis Model Mech 13:N/A (2020). PubMed: 31848207
    • Lau A  et al. a-Synuclein strains target distinct brain regions and cell types. Nat Neurosci 23:21-31 (2020). PubMed: 31792467
    • Sheng L  et al. Erythrocytic a-synuclein contained in microvesicles regulates astrocytic glutamate homeostasis: a new perspective on Parkinson's disease pathogenesis. Acta Neuropathol Commun 8:102 (2020). PubMed: 32641150
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-Alpha-synuclein antibody [MJFR1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (SHSY5Y cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycin gel)
    Loading amount
    40 µg
    Specification
    SHSY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 26 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Alpha-synuclein antibody [MJFR1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Miss. Maria Poley

    Verified customer

    Submitted Nov 06 2020

    Western blot abreview for Anti-Alpha-synuclein antibody [MJFR1]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SH-SY5Y cells)
    Gel Running Conditions
    Reduced Denaturing (8-12% gradient gel)
    Loading amount
    10 µg
    Specification
    SH-SY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 08 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Alpha-synuclein antibody [MJFR1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (synuclein inclusion in human frozen brain section)
    Permeabilization
    Yes - 0.2% triton in PBS
    Specification
    synuclein inclusion in human frozen brain section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde + methanol
    Read More

    Diego Perez Rodriguez

    Verified customer

    Submitted Dec 03 2018

    Western blot abreview for Anti-Alpha-synuclein antibody [MJFR1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Recombinant protein (Human and transgenic mouse M83)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    1 µg
    Specification
    Human and transgenic mouse M83
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Sophie Morgan

    Verified customer

    Submitted Jun 29 2018

    Western blot abreview for Anti-alpha Synuclein antibody [MJFR1]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10% Gel)
    Sample
    Human Cell lysate - whole cell (SH-SY5Y Neuroblastoma cell line)
    Specification
    SH-SY5Y Neuroblastoma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Fredrik Hoel

    Verified customer

    Submitted Dec 16 2014

    Question

    Is it possible to obtain the concentration of your anti-alpha-synuclein clone MJFR1 (ab138501)?

    Read More

    Abcam community

    Verified customer

    Asked on May 02 2013

    Answer



    ab138501 is an unpurified tissue culture supernatant product, so the concentration of the specific anti-alpha Synuclein antibody is not determined. However in general, for products of this type, the specific antibody concentration is about 0.05mg/ml.

    Read More

    Abcam Scientific Support

    Answered on May 02 2013

    Question

    We would be interested in buying the Human Synuclein antibody [ab138501] in case it is guaranteed that the antibody recognises specifically human synuclein but not rat synuclein.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2013

    Answer


    I have conducted a sequence comparison between the human and rat alpha synuclein proteins and there is a very high sequence homology. Out of the 140 amino acids of this protein, 133 amino acids are shared between human and rat which suggests that it is unlikely that any antibody you will find to alpha-synuclein will definitely not cross-react with rat since there is no sequence within this protein that varies significantly between rat and human.

    ab138501 has been tested in mouse, rat and human and only showed positive results in mouse and human. This does not mean that it will not work in rat since with some optimisation, the rat protein could also be detected.

    However, since our datasheet specifically states that this antibody does not work in rat, we will honour our guarantee and if you buy and test this antibody in rat and find that it works, we will refund or replace the antibody.

    Read More

    Abcam Scientific Support

    Answered on Mar 19 2013

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