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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein
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Validated using a knockout cell line

Anti-Alpha-synuclein antibody [syn204] (ab3309)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (8)

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Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)

    Key features and details

    • Mouse monoclonal [syn204] to Alpha-synuclein
    • Suitable for: ICC
    • Knockout validated
    • Reacts with: Human
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Product image
    Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)
    Peptide
    Alpha-synuclein (phospho S129) peptide (ab188826)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Alpha-synuclein antibody [syn204]
      See all Alpha-synuclein primary antibodies
    • Description

      Mouse monoclonal [syn204] to Alpha-synuclein
    • Host species

      Mouse
    • Specificity

      Due to sequence homology ab3309 might react with Beta synuclein
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Recombinant full length protein (Human).

    • Positive control

      • ICC: HAP1 cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      syn204
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Synuclein
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3309 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    ICC
    Use a concentration of 10 µg/ml.
    Notes
    ICC
    Use a concentration of 10 µg/ml.

    Target

    • Function

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificity

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in disease

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similarities

      Belongs to the synuclein family.
    • Domain

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational
      modifications

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localization

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Target information above from: UniProt accession P37840 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6622 Human
      • Omim: 163890 Human
      • SwissProt: P37840 Human
      • Unigene: 21374 Human
      • Alternative names

        • Alpha synuclein antibody
        • Alpha-synuclein antibody
        • Alpha-synuclein, isoform NACP140 antibody
        • alphaSYN antibody
        • MGC105443 antibody
        • MGC110988 antibody
        • MGC127560 antibody
        • MGC64356 antibody
        • NACP antibody
        • Non A beta component of AD amyloid antibody
        • Non A4 component of amyloid antibody
        • Non A4 component of amyloid precursor antibody
        • Non-A beta component of AD amyloid antibody
        • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
        • Non-A4 component of amyloid precursor antibody
        • Non-A4 component of amyloid, precursor of antibody
        • OTTHUMP00000218549 antibody
        • OTTHUMP00000218551 antibody
        • OTTHUMP00000218552 antibody
        • OTTHUMP00000218553 antibody
        • OTTHUMP00000218554 antibody
        • PARK 1 antibody
        • PARK 4 antibody
        • PARK1 antibody
        • PARK4 antibody
        • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
        • Parkinson disease familial 1 antibody
        • SNCA antibody
        • Snca synuclein antibody
        • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYN antibody
        • Synuclein alpha antibody
        • Synuclein alpha 140 antibody
        • Synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYUA_HUMAN antibody
        see all

      Images

      • Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)
        Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)
        ab3309 staining Alpha-Synuclein in wild-type Hap1 cells (top panel) and SNCA knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab3309 at 10μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
        Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab3309? Please let us know so that we can cite the reference in this datasheet.

      ab3309 has been referenced in 8 publications.

      • Park HJ  et al. Ethanol extract from Gynostemma pentaphyllum ameliorates dopaminergic neuronal cell death in transgenic mice expressing mutant A53T human alpha-synuclein. Neural Regen Res 15:361-368 (2020). PubMed: 31552910
      • Agerschou ED  et al. An engineered monomer binding-protein for a-synuclein efficiently inhibits the proliferation of amyloid fibrils. Elife 8:N/A (2019). PubMed: 31389332
      • Nevzglyadova OV  et al. Yeast red pigment modifies cloned human a-synuclein pathogenesis in Parkinson disease models in Saccharomyces cerevisiae and Drosophila melanogaster. Neurochem Int 120:172-181 (2018). PubMed: 30099122
      • Ma L  et al. C-terminal truncation exacerbates the aggregation and cytotoxicity of a-Synuclein: A vicious cycle in Parkinson's disease. Biochim Biophys Acta Mol Basis Dis 1864:3714-3725 (2018). PubMed: 30290273
      • Shahnawaz M  et al. Development of a Biochemical Diagnosis of Parkinson Disease by Detection of a-Synuclein Misfolded Aggregates in Cerebrospinal Fluid. JAMA Neurol 74:163-172 (2017). PubMed: 27918765
      • Bassil F  et al. Reducing C-terminal truncation mitigates synucleinopathy and neurodegeneration in a transgenic model of multiple system atrophy. Proc Natl Acad Sci U S A 113:9593-8 (2016). SDS-PAGE . PubMed: 27482103
      • Volpicelli-Daley LA  et al. Addition of exogenous a-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous a-synuclein to Lewy body and Lewy neurite-like aggregates. Nat Protoc 9:2135-46 (2014). PubMed: 25122523
      • Croisier E  et al. Comparative study of commercially available anti-alpha-synuclein antibodies. Neuropathol Appl Neurobiol 32:351-6 (2006). PubMed: 16640654

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Thank you very much. Could you please let me know whether I can use PBS buffer as antibody diluent( my second query of ref:914295)

      Read More

      Abcam community

      Verified customer

      Asked on Oct 04 2006

      Answer

      Thank you for getting back to me. Phosphate buffered saline can be used as an antibody diluent as an alternative to TBS for western blot analysis. However, with regards storage I would recommend that the antibodies are alituotted and frozen at -20oC upon arrival without prior dilution. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Oct 05 2006

      Question

      Do you know this antibody's epitope, ie what stretch of amino acids of synuclein that it binds to?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 13 2003

      Answer

      No I am afraid we do not know the epitope for this product, we will update the on line datasheet if we receive any further information.

      Read More

      Asdf Edo

      Abcam Scientific Support

      Answered on Oct 13 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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