Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
None
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Genemed Acu-StainTM Mouse + Rabbit HRP Kits
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Abcam response
We are sorry that this antibody did not provide satisfactory results for this customer.
This antibody has previously been tested in rat species with negative results. Therefore, as stated on the datasheet, we do not recommend this antibody in rat samples and rat species is not covered by our guarantee. We do have on our catalogue Anti-alpha Synuclein antibodies that have been tested (and therefore guarantee) in rat and IHC-P. Please do not hesitate to contact our Scientific Support team if you have any questions.
Abcam user community
Verified customer
Submitted Jul 23 2014