Product nameAnti-Alpha-synuclein (phospho S129) antibody
See all Alpha-synuclein primary antibodies
DescriptionRabbit polyclonal to Alpha-synuclein (phospho S129)
SpecificityThis antibody is specific for the 15 kDa alpha Synuclein protein phosphorylated at serine 129. Immunolabelling is blocked by the phosphopeptide used as an antigen but not by the corresponding dephosphopeptide.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog, Non human primates
Synthetic peptide corresponding to Rat Alpha-synuclein. Phosphopeptide corresponding to amino acid residues surrounding the phospho serine 129 of Rat alpha Synuclein.
- Rat cortex lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from rabbit serum by affinity purification via sequential chromatography on phosph- and dephosphopeptide affinity columns.
Our Abpromise guarantee covers the use of ab42906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 15 kDa.|
FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body
Sequence similaritiesBelongs to the synuclein family.
DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
- Information by UniProt
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab42906) at 1/1000 dilution
Lane 1 : Rat cortex lysate at 20 µg
Lane 2 : Rat cortex lysate with immunogenic peptide
Predicted band size: 15 kDa
Western blot of rat cortex lysate showing specific labelling of the ~15kDa alpha Synuclein protein phosphorylated at serine 129, using ab42906 at a 1/1000 dilution. Immunolabeling is blocked by the phosphopeptide (peptide) used as immunogen.
This product has been referenced in:
- Toba S et al. Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport. Sci Rep 7:16386 (2017). Read more (PubMed: 29180624) »
- Stoica G et al. Potential role of a-synuclein in neurodegeneration: studies in a rat animal model. J Neurochem 122:812-22 (2012). Read more (PubMed: 22639889) »