Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho S129)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Alpha-synuclein (phospho S129) antibody
See all Alpha-synuclein primary antibodies
DescriptionRabbit polyclonal to Alpha-synuclein (phospho S129)
Detects endogenous levels of Synuclein only when phosphorylated at serine 129. Due to 69% sequence homology ab59264 might react with Beta synuclein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Alpha-synuclein. Synthetic phosphopeptide derived from Human Synuclein around the phosphorylation site of serine 129 (M-P-SP-E-E).
Database link: P37840
- Human and mouse brain.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab59264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.
Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes.
Primary antibody incubation: 1 hour at 37°C
Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes.
|WB||1/500 - 1/1000.
Please see WB protocol details in the image legend.
FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body
Sequence similaritiesBelongs to the synuclein family.
DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
- Information by UniProt
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/500 dilution
Lane 1 : Mouse brain whole cell lysates
Lane 2 : Mouse brain whole cell lysates with immunogen phosphopeptide
Lysates/proteins at 40 µg per lane.
Blocking buffer: 5% (w/v) BSA in TBST.
Primary antibody dilution buffer: 5%(w/v)BSA,0.1%(v/v), Tween-20 in TBST.
Secondary antibody dilution buffer: 5%(w/v)BSA,0.1%(v/v),Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59264 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/1000 dilution
Lanes 1-3 : Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein
Lanes 4-6 : Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein
Lane 7 : Whole tissue lysate prepared from KO mouse
Lysates/proteins at 50 µg per lane.
All lanes : Goat anti-rabbit Ig (H+L) HRP at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 18,19 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
ab59264 has been referenced in 41 publications.
- Courte J et al. The expression level of alpha-synuclein in different neuronal populations is the primary determinant of its prion-like seeding. Sci Rep 10:4895 (2020). PubMed: 32184415
- Tang L et al. Effect of Wenshen-Yanggan Decoction on Movement Disorder and Substantia Nigra Dopaminergic Neurons in Mice with Chronic Parkinson's Disease. Evid Based Complement Alternat Med 2020:9838295 (2020). PubMed: 32655672
- McGlinchey RP et al. C-terminal a-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem 294:9973-9984 (2019). PubMed: 31092553
- Diniz LP et al. a-synuclein oligomers enhance astrocyte-induced synapse formation through TGF-ß1 signaling in a Parkinson's disease model. J Neurochem 150:138-157 (2019). PubMed: 31009074
- Shahmoradian SH et al. Lewy pathology in Parkinson's disease consists of crowded organelles and lipid membranes. Nat Neurosci 22:1099-1109 (2019). PubMed: 31235907