Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)
- Datasheet
- References (69)
- Protocols
Overview
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Product nameAnti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
See all Alpha-synuclein primary antibodies -
DescriptionRabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129)
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Host speciesRabbit
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SpecificityThis antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.
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Tested applicationsSuitable for: IHC-FrFl, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-Fr or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
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Positive control
- IHC-P: Human Parkinson Substantia Nigra tissue. WB: Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein; Recombinant alpha-synuclein phosphorylated at S129.
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General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1536Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab51253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FrFl | 1/5000. | |
| WB | 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
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Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
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Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similaritiesBelongs to the synuclein family.
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DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
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Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
- Information by UniProt
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Database links
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 21374 Human
see all -
Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
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![Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)](https://www.abcam.com/ps/products/51/ab51253/Images/ab51253-271808-ab51253.jpg)
Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)This image is courtesy of Professor Michael Goedert’s team (Sophie Morgan and Masami Masuda-Suzukake).All lanes : Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1 : Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein (line M83)
Lane 2 : Recombinant alpha-synuclein phosphorylated at S129, 3ng
Predicted band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)](https://www.abcam.com/ps/products/51/ab51253/Images/ab51253-261060-ab51253YL120314PSHuBrainParkin10ugDATASHEET.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51253, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)](https://www.abcam.com/ps/products/51/ab51253/Images/ab51253-341600-ab5125364512.jpg)
Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)This image is courtesy of an abreview by Sophie MorganIHC image of alpha Synuclein (phospho S129) staining in free floating tgM83+/- or tgM83+/+ mouse brain tissue. The section was incubated with ab51253, 1/5000, for 15 hours at 4oC and detected using a Biotinylated conjugated Anti-Rabbit monocloal antibody, 1/200.
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![ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)](https://www.abcam.com/ps/products/51/ab51253/Images/ab51253-255509-51253-ELISA.jpg)
ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)Direct ELISA antibody dose-response curve using ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
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![Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)](https://www.abcam.com/ps/products/51/ab51253/Images/ab51253-255510-51253-Dot.jpg)
Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
References
This product has been referenced in:
- Refolo V et al. Progressive striatonigral degeneration in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapies. Acta Neuropathol Commun 6:2 (2018). Read more (PubMed: 29298733) »
- Rusconi R et al. Long-lasting pathological consequences of overexpression-induced a-synuclein spreading in the rat brain. Aging Cell 17:N/A (2018). IHC-FrFl ; Rat . Read more (PubMed: 29383832) »
