• Product name
    Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
    See all Alpha-synuclein primary antibodies
  • Description
    Rabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129)
  • Host species
  • Specificity
    This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.
  • Tested applications
    Suitable for: IHC-FrFl, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-Fr or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Alpha-synuclein aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P37840
    (Peptide available as ab188826)

  • Positive control
    • IHC-P: Human Parkinson Substantia Nigra tissue. WB: Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein; Recombinant alpha-synuclein phosphorylated at S129.
  • General notes



    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab51253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/5000.
WB 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-Fr or IP.
  • Target

    • Function
      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • Tissue specificity
      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • Involvement in disease
      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • Sequence similarities
      Belongs to the synuclein family.
    • Domain
      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • Post-translational
      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • Cellular localization
      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha synuclein antibody
      • Alpha-synuclein antibody
      • Alpha-synuclein, isoform NACP140 antibody
      • alphaSYN antibody
      • MGC105443 antibody
      • MGC110988 antibody
      • MGC127560 antibody
      • MGC64356 antibody
      • NACP antibody
      • Non A beta component of AD amyloid antibody
      • Non A4 component of amyloid antibody
      • Non A4 component of amyloid precursor antibody
      • Non-A beta component of AD amyloid antibody
      • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
      • Non-A4 component of amyloid precursor antibody
      • Non-A4 component of amyloid, precursor of antibody
      • OTTHUMP00000218549 antibody
      • OTTHUMP00000218551 antibody
      • OTTHUMP00000218552 antibody
      • OTTHUMP00000218553 antibody
      • OTTHUMP00000218554 antibody
      • PARK 1 antibody
      • PARK 4 antibody
      • PARK1 antibody
      • PARK4 antibody
      • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
      • Parkinson disease familial 1 antibody
      • SNCA antibody
      • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYN antibody
      • Synuclein alpha antibody
      • Synuclein alpha 140 antibody
      • Synuclein, alpha (non A4 component of amyloid precursor) antibody
      • SYUA_HUMAN antibody
      see all


    • All lanes : Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

      Lane 1 : Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein (line M83)
      Lane 2 : Recombinant alpha-synuclein phosphorylated at S129, 3ng

      Predicted band size: 14 kDa

    • IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51253, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.

       For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • IHC image of alpha Synuclein (phospho S129) staining in free floating tgM83+/- or tgM83+/+ mouse brain tissue. The section was incubated with ab51253, 1/5000, for 15 hours at 4oC and detected using a Biotinylated conjugated Anti-Rabbit monocloal antibody, 1/200. 

      See Abreview

    • Direct ELISA antibody dose-response curve using ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    • Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.


    This product has been referenced in:
    • Refolo V  et al. Progressive striatonigral degeneration in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapies. Acta Neuropathol Commun 6:2 (2018). Read more (PubMed: 29298733) »
    • Rusconi R  et al. Long-lasting pathological consequences of overexpression-induced a-synuclein spreading in the rat brain. Aging Cell 17:N/A (2018). IHC-FrFl ; Rat . Read more (PubMed: 29383832) »
    See all 69 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Q&A


    1)Our initial testing with rat showed negative and that the antibody is not recommended for use in rat. However, based on sequence analysis, it shares 100% identity with rat.

    2)We previously tested rat brain, heart, kidney and spleen tissues lyates.

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    Thank you for contacting us.

    I am sorry to hear of the trouble which you have been experiencing when lot no. GR93844. We have not seen this issue with other lots of ab51253. This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.

    I do hope that you are able to resolve whether this is an antibody or sample issue.If you find that the issue is with the antibody please do not hesitate to contact our Scientific Support group at technical@abcam.com who will be very happy to help you resolve any issue with this product.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!


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    Merci pour votre réponse.

    Nous sommes très désolé de cette inconvenance. Veuillez croire que la qualité de nos produits est très important pour nous. J'ai transmis votre feedback aussi au laboratoire.

    Nous avons actuellement trois autres tube du même lot en stock. Est-ce que ce lot vous convient et est-ce que vous souhaitez trois tubes de celui-ci? Autrement, je peux aussi vous envoyer des anticorps alternatives, contre une autre protéine,si vous souhaitez. Vous avez le droit à trois tubes d'anticorps et c'est votre choix lesquels vous souhaitez.

    Veuillez ne pas hésiter à me recontacter si vous avez des questions. Je me réjouis de votre réponse

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    Merci beaucoup pour votre patience pendant que j'étais en correspondance avec le laboratoire.

    Effectivement, le laboratoire avait obtenu en signal faible avec ce lot à 1/500 et nous allons donc vous remplacer les deux dernier tubes. Veuillez s'il vous plaît me confirmer avec combien des tubes vous avez eu ce problème exactement.
    J'ai vu que vous avez placé une commande pour deux autres tubes qui devrait arriver prochainement chez vous. Est-ce que je vous envoie directement ces deux tubes gratuitement ou est-ce que vous préférez des autres tubes ou un avoir?

    Le laboratoire m'avait aussi confirmé un utilisateur avait utilisé tous les lots en Western blot et avait obtenu un bon signal avec tous les lots, mais seulement en utilisant une technique d'optimisation. Effectivement, le laboratoire recommande de prétraiter la membrane Western blot après transfert avec 0.4% de PFA pour 30 minutes avant de bloquer avec du lait. J’ai aussi ajouté une note pour ceci sur notre fiche technique.

    J'espère que ceci peux améliorer aussi vos résultats.

    Veuillez me confirmer s'il vous plaît comment vous souhaitez procéder par rapport au remboursement/remplacement. Veuillez ne pas hésiter non plus à nous recontacter si vous avez des autres questions.

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    Vielen Dank für Ihren Anruf.

    Ich freue mich sehr, Ihnen gute Nachrichten überbringen zu können.

    Wir werdenab Ende der nächsten Woche ab51253 (Anti-alpha Synuclein (phospho S129) antibody [EP1536Y]) wieder anbieten können.

    Wir freuen uns auf Ihre Bestellung und wünschen Ihnen viel Erfolg in Ihrer Forschung.

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