Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-alpha Synuclein (phospho S129) antibody [EP1536Y]

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Human Tissue sections (PD patient brain sections, mesencephalon, 40 um)
Specification
PD patient brain sections, mesencephalon, 40 um
Permeabilization
Yes - 0.5% Triton-X 100
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in TBS with 0.5% Triton-X 100
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Biotinylated anti-rabbit IgG (H+L)
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400

Additional data

Additional Notes
4% PFA fixation. This antibody provides low background and good specific staining of Lewy bodies and Lewy neurites (was compared to clone LB 509).

Miss. Oldriska Chutna

Verified customer

Submitted May 30 2014

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