Western blot abreview for Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - other (Human brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris gel)
Loading amount
1 µg
Specification
Human brain lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/4000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)-HRP conjugate
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 14 kDa

Sophie Morgan

Verified customer

Submitted May 16 2018

Sign up