Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho Y125)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Alpha-synuclein (phospho Y125) antibody
See all Alpha-synuclein primary antibodies
DescriptionRabbit polyclonal to Alpha-synuclein (phospho Y125)
Specificityab131466 detects alpha Synuclein only when phosphorylated at tyrosine 125.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Alpha-synuclein conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH, surrounding phosphorylation site of Tyrosine 125 (E-A-Yp-E-M) derived from Human alpha Synuclein (NP_000336.1).
- HeLa cells; extracts of C2C12 cells treated with anisomycin.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131466 was purified by affinity- chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab131466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).|
|ICC/IF||1/100 - 1/200.|
FunctionMay be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Tissue specificityExpressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
Involvement in diseaseGenetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body
Sequence similaritiesBelongs to the synuclein family.
DomainThe 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
Cellular localizationCytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
- Information by UniProt
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
All lanes : Anti-Alpha-synuclein (phospho Y125) antibody (ab131466) at 1/500 dilution
Lane 1 : Extract from C2C12 cells, untreated
Lane 2 : Extract from C2C12 cells treated with Anisomycin
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunoflourescent analysis of alpha Synuclein (phospho Y125)in methanol fixed HeLa cells stained with ab131466 at a 1/100 dilution.
ab131466 has not yet been referenced specifically in any publications.