Anti-Alpha-synuclein (phospho Y136) antibody (ab131491)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho Y136)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Alpha-synuclein (phospho Y136) antibody
See all Alpha-synuclein primary antibodies -
Description
Rabbit polyclonal to Alpha-synuclein (phospho Y136) -
Host species
Rabbit -
Specificity
ab131491 detects endogenous levels of alpha Synuclein only when phosphorylated at tyrosine 136. Due to 72.7% sequence homology ab131491 might react with Beta synuclein -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Alpha-synuclein aa 100-200 (phospho Y136) conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH, surrounding phosphorylation site of Tyrosine 136 (Q-D-Y(p)-E-P) derived from Human alpha synuclein (NP_000336.1)
-
Positive control
- Mouse brain tissue lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab131491 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 14 kDa.
|
|
ICC/IF |
1/100 - 1/200. (methanol fixed)
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 14 kDa. |
ICC/IF
1/100 - 1/200. (methanol fixed) |
Target
-
Function
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
Tissue specificity
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
Involvement in disease
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
Sequence similarities
Belongs to the synuclein family. -
Domain
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
Post-translational
modificationsPhosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
Cellular localization
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
-
Database links
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- Unigene: 21374 Human
- Unigene: 17484 Mouse
-
Alternative names
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
Images
-
Immunofluorescence analysis of methanol-fixed HeLa cells labelling alpha Synuclein using ab131491, at 1/100 dilution.
-
All lanes : Anti-Alpha-synuclein (phospho Y136) antibody (ab131491) at 1/500 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse brain tissue lysate with blocking peptide
Predicted band size: 14 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab131491 has not yet been referenced specifically in any publications.