Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Acetylated alpha Tubulin antibody [6-11B-1]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate Carcinoma)
Specification
Prostate Carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Retrieval buffer
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: DMEM with 10% CS FBS

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti mouse - Alexa Fluor 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500

Additional data

Additional Notes
These sections were created from archived clinical specimens. Works well with paraffin, even very old blocks.

Abcam user community

Verified customer

Submitted Sep 30 2009

Sign up