Immunocytochemistry/ Immunofluorescence abreview for Anti-Acetylated alpha Tubulin antibody [6-11B-1]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (Mouse Glioblastoma (GL261))
Permeabilization
Yes - 0.3% TritonX-100 in 1X TBS
Specification
Mouse Glioblastoma (GL261)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 28°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in 1XTBS with 0.1% TritonX-100
Dilution
1/1000

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat Anti-mouse IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
For fixation 4% Paraformaldehyde was used.

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Submitted Sep 16 2014

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