Immunocytochemistry/ Immunofluorescence abreview for Anti-Acetylated alpha Tubulin antibody [6-11B-1]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Monkey Cell (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
3% PFA and 0.1% GA

Other product details

Incubation time
45 minute(s) · Temperature: 25°C · Diluent: 3% BSA and 0.5% TX100
Dilution
1/100

Secondary antibody

Concentration
2 µg/ml
Name
Non-Abcam antibody was used: Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 647

Additional data

Additional Notes
Acetylated tubulin is displayed in green. Tyrosinated tubulin was stained with Rat anti Tubulin (YL1/2) antibody and is displayed in magenta. The sample was extracted prior to fixation with 0.5% TX100 in 1x PEM buffer for 20s.

Dr. Aaron Halpen

Verified customer

Submitted Sep 29 2014

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