Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [6-11B-1]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (immortalised bone marrow-derived macrophage)
Permeabilization
Yes - 0.1% Triton X-100
Specification
immortalised bone marrow-derived macrophage
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Block buffer
Dilution
1/200

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: anti-mouse Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568

Additional data

Additional Notes
Cells were stained with Abcam anti-acetylated alpha-tubulin (red) and anti-alpha-tubulin (non-Abcam, green)

Dr. James Harris

Verified customer

Submitted Jun 02 2017

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