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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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RecombinantRabMAb

Recombinant Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

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  • Certificate of Compliance
Reviews (4) Submit a question References (13)

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Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Flow Cytometry - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
  • Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16772] to alpha Tubulin (acetyl K40)
  • Suitable for: WB, ICC/IF, Flow Cyt, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR16772] to alpha Tubulin (acetyl K40)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, C6 and NIH/3T3 whole cell lysates (treated with 500 ng/ml Trichostatin A for 4 hours); Mouse brain, kidney and spleen lysates; Rat brain and heart lysates; Human fetal heart and fetal kidney lysates. IHC-P: Human and Mouse cerebral cortex tissue; rat cerebellum tissue. IF: HeLa cells treated with 50 ug/ml Trichostatin A for 4 hours. Flow: HeLa cells treated with 500ng/ml Trichostatin A for 4 hours. IP: HeLa treated with 500 ng/ml Trichostatin A for 4 hours.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16772
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Associated products

  • Alternative Versions

    • Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (ab209348)
    • Alexa Fluor® 647 Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab218591)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC-P
Mouse
Rat
Human
IP
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
ICC/IF (1)
1/500.
Flow Cyt
1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP
1/70.
IHC-P (3)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
ICC/IF
1/500.
Flow Cyt
1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP
1/70.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    ab179484 stained in Hela cells. Untreated and Trichostatin A treated (50ug/ml, 4 hours) cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab179484 at 1/500 dilution overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on Purkinje cells of cerebellum. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    All lanes : Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Human fetal heart lysate
    Lane 7 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    All lanes : Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/20000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 500 ng/ml Trichostatin A for 4 hours
    Lane 2 : Untreated HeLa whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Ab179484 staining alpha Tubulin in HFF-1 (Human skin fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:20000 dilution. An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain at 1:200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing cilia (arrows) staining in HFF-1 cells treated with starvation for 48 hours.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Ab179484 staining alpha Tubulin in NIH/3T3 (mouse embryonic fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence).The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:20000 dilution. An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain at 1:200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing cilia (arrows) staining in NIH/3T3 cells treated with starvation for 48 hours.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Ab179484 staining alpha Tubulin in NIH/3T3 (mouse embryonic fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:20000 dilution. An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain at 1:200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing midbody (arrows) staining in NIH/3T3 cells treated with starvation for 48 hours.

  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    All lanes : Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/20000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate treated with 500 ng/ml Trichostatin A for 4 hours
    Lane 2 : Untreated C6 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Western blot - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    All lanes : Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484) at 1/20000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate treated with 500 ng/ml Trichostatin A for 4 hours
    Lane 2 : Untreated NIH/3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    Lane 2 : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on neuron cells of Human brain tissue. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on neuron cells of Mouse cerebral cortex tissue. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Flow Cytometry - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells treated with 500 ng/ml Trichostatin A for 4 hours labeling alpha Tubulin (acetyl K40) with ab179484 at 1/240 dilution (red line). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.  ab179484 preincubated with 1mg/ml acetyl Alpha tubulin (acetyl K40) peptide (green) or non-acetyl Alpha tubulin (acetyl K40) peptide (orange). The isotype control was Rabbit monoclonal IgG (black) and the unlabelled contol was cells without incubation with primary antibody and secondary antibody (blue).

  • Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

    Alpha Tubulin was immunoprecipitated from 1mg of HeLa cells (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours with ab179484 at 1/70 dilution. Western blot was performed from 10 µg of the immunoprecipitate using ab179484 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Hela whole cell extract. Right lane: PBS instead of Hela whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)
    Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (ab179484)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (13)

    Publishing research using ab179484? Please let us know so that we can cite the reference in this datasheet.

    ab179484 has been referenced in 13 publications.

    • Albashari A  et al. Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury. ACS Omega 5:16064-16075 (2020). PubMed: 32656428
    • Bhaskar A  et al. Host sirtuin 2 as an immunotherapeutic target against tuberculosis. Elife 9:N/A (2020). PubMed: 32697192
    • Ochi T  et al. CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions. Structure 28:674-689.e11 (2020). PubMed: 32375023
    • Dumm RE  et al. Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function. Nat Commun 10:779 (2019). PubMed: 30770807
    • Yang W  et al. A novel nuclear factor Akirin regulating the expression of antimicrobial peptides in Chinese mitten crab Eriocheir sinensis. Dev Comp Immunol 101:103451 (2019). PubMed: 31306698
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Bos taurus Tissue sections (oviduct)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
    Permeabilization
    No
    Specification
    oviduct
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 04 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (adult mouse lung)
    Specification
    adult mouse lung
    Permeabilization
    Yes - 0.5% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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