Overview

  • Product name
    Anti-alpha Tubulin antibody
    See all alpha Tubulin primary antibodies
  • Description
    Rabbit polyclonal to alpha Tubulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Pig, Drosophila melanogaster, Zebrafish
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
  • Immunogen

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PEY2

  • Positive control
    • IHC-P: Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab15246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 17785506
IHC-Fr Use at an assay dependent concentration. PubMed: 21069788
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.

    This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).

     

  • All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Ladds MJGW  et al. Autophagic flux blockage by accumulation of weakly basic tenovins leads to elimination of B-Raf mutant tumour cells that survive vemurafenib. PLoS One 13:e0195956 (2018). Read more (PubMed: 29684045) »
  • Jahandideh F  et al. Purification and identification of adipogenic-differentiating peptides from egg white hydrolysate. Food Chem 259:25-30 (2018). WB ; Mouse . Read more (PubMed: 29680051) »
See all 131 Publications for this product

Customer reviews and Q&As

1-10 of 23 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 08 2018

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse Glioblastoma (GL261))
Specification
Mouse Glioblastoma (GL261)
Treatment
HDAC inhibitors for 24hrs
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Sep 12 2014

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (12.5)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted May 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 15°C

Ms. AeLee Jeong

Verified customer

Submitted Apr 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (High-fat diet-fed SD rat muscle)
Loading amount
30 µg
Specification
High-fat diet-fed SD rat muscle
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 18 2013

Question
Answer

We would recommend the use of one of the following products for a loading control with zebrafish samples: AB15224 anti alpha Tubulin (https://www.abcam.com/alpha-tubulin-antibody-ab15246.html). This product has been featured in the following publication for use in WB on zebrafish (PubMed ID: 22581286). AB6046 anti beta Tubulin. This product has an excellent Abreview provided by a customer using in WB on zebrafish. This review is located at the following web address: https://www.abcam.com/beta-Tubulin-antibody-Loading-Control-ab6046-reviews.html?intabreviewid=15893 AB14744 anti- COX IV antibody (https://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-Control-ab14744-references.html). Another guaranteed product for use as loading control this product has been used in publication (PubMed ID: 18765295).

Read More

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab15246 in plasmodium falciparum. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for plasmodium falciparumand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in ab15246.

To our knowledge, ab15246has not been tested in plasmodium falciparum. Therefore, I can offer a discount off a future purchase if you buy ab15246 now, test it in plasmodium falciparum and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab15246 in plasmodium falciparum. I will then send a discount code. This code must be issued before purchasing ab15246 so please wait for my reply before ordering.

2. Purchase ab15246either by phone, fax, or online (www.abcam.com).

3. Test it in plasmodium falciparum.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15246 turns out to be unsuitable for plasmodium falciparum, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 T)
Total protein in input
100 µg
Specification
HEK 293 T
Immuno-precipitation step
Protein A/G

Dr. Chris Tracy

Verified customer

Submitted Sep 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Loading amount
100000 cells
Specification
Hela cells
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 23 2012

1-10 of 23 Abreviews or Q&A

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