Overview

  • Product name

    Anti-alpha Tubulin antibody
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit polyclonal to alpha Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human, Pig, Drosophila melanogaster, Zebrafish
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
  • Immunogen

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PEY2

  • Positive control

    • SK N SH whole cell lysate (ab3956) can be used as a positive control in WB. IHC-P: Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab15246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 17785506
IHC-Fr Use at an assay dependent concentration. PubMed: 21069788
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.

    This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).

     

  • All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Nardi F  et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep 9:3035 (2019). Read more (PubMed: 30816200) »
  • Toyoda Y  et al. Functional Characterization of Clinically-Relevant Rare Variants in ABCG2 Identified in a Gout and Hyperuricemia Cohort. Cells 8:N/A (2019). Read more (PubMed: 31003562) »
See all 153 Publications for this product

Customer reviews and Q&As

11-20 of 25 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 T)
Total protein in input
100 µg
Specification
HEK 293 T
Immuno-precipitation step
Protein A/G

Dr. Chris Tracy

Verified customer

Submitted Sep 18 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Loading amount
100000 cells
Specification
Hela cells
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 23 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
10 µg
Specification
HEK 293T
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Blocking buffer (LiCor, 927-40003) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 20 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (Hek 293)
Loading amount
10 µg
Specification
Hek 293
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
1:1 PBS/Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C

Dr. Chris Tracy

Verified customer

Submitted Jan 12 2012

Answer

Thank you for contacting us. We would guarantee both ab15246 and ab18251 to stain cells from drosophila via immunocytochemistry (ICC/IF). I am not sure why drosophila wasn't added as a tested species to the datasheet for ab18251 after that Abreview was added, but this was an oversight and I have since updated the datasheet to include drosophila as a tested species. As for the references for ab1546, we know of them testing drosophila by WB and IHC-Fr, not ICC/IF, per se. But we would still guarantee the antibody to work in drosophila and ICC/IF since both the species and application were tested, although independently. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048163/pdf/cm0068-0001.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562427/pdf/nihms70131.pdf If you're looking for an ICC/IF protocol to use with ab1546, you can look at the below two articles, tested in mouse and human, and just apply that same protocol to your cells from drosophila. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872425/pdf/pnas.200910941.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851247/pdf/nihms120363.pdf Both of these antibodies would be covered under our Abpromise to use them with drosophila in ICC/IF so if you ran into any difficulty, we would be happy to replace or refund them for you within 6 months of your purchase. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you once again for your enquiry and for your patience. I am sorry to confirm that for Beta tubulin, the antibodies we have available tested and guaranteed in EM and mouse, rat and human tissues are mouse monoclonals. I can suggest to consider trying the following rabbit monoclonal antibody which is tested in mouse, however I am sorry this is not tested and guaranteed for EM. ab52623 Rabbit mono Anti-beta Tubulin antibody [EP1569Y] Applications Flow Cyt, ICC, IHC-P, IP, WB Reacts with: Human, Mouse, Rat Immunogen propriatory, but it is right on the C-terminus and about 15 residues long. https://www.abcam.com/index.html?datasheet=52623 https://www.abcam.com/index.html?datasheet=52623. For alpha tubulin, we have the following antibody tested in EM and mouse, rat and human but rasied in mouse. I am sorry the epitope has not been mapped: ab80779 Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free Mouse monoclonal Applications: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse, Rat, Chicken, Cow, Gerbil, Guinea pig, Pig, Xenopus laevis https://www.abcam.com/index.html?datasheet=80779 https://www.abcam.com/index.html?datasheet=80779. I understand you concerns that mouse on mouse can sometimes give non specific binding. However, this has been tested succesfully in mouse, and we are able to provide our guarantee for the results from mouse and EM. Otherwise, it could be preferable to try an antibody raised in a different species. The following rabbit polyclonal is tested in mouse, rat and human and is raised against a peptide from the C terminus. However, I am sorry it is not tested and guaranteed in EM: ab15246 Anti-alpha Tubulin antibody Rabbit polyclonal Applications: ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Pig, Rat, Zebrafish Immunogen: ALEKDYEEVGVDSVEGEGEEEGEEY C terminal amino acids 426-450 of Human alpha Tubulin https://www.abcam.com/index.html?datasheet=15246 https://www.abcam.com/index.html?datasheet=15246. With regards to the search facility on the website, I understand it is not possible to search by immunogen or region. This is partly because although we produce many antibodies in house and can provide the immunogen sequence, we do source many externally and sometimes the immunogen information is propriatory (commercially sensitive). We can usually provide this information on individual customer request. Thank you for your understanding. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry. I can confirm that the loading control protein alpha tubulin should only be located in the cytoplasm. We have several antibodies for detection of alpha tubulin which have been tested in WB, human and pig. I can recommend to review the datasheets for further information. ab15246 Anti-alpha Tubulin antibody Rabbit polyclonal Applications: ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Pig, Rat, Zebrafish Click here (or use the following: https://www.abcam.com/index.html?datasheet=15246). ab7750 Anti-alpha Tubulin antibody [TU-01] Mouse monoclonal Applications: ELISA, Flow Cyt, ICC, IF, IHC-P, IP, WB Reacts with: Human, Mouse, Arabidopsis thaliana, Cow, Dog, Pig Click here (or use the following: https://www.abcam.com/index.html?datasheet=7750). ab80779 Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free Mouse monoclonal Applications: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse, Rat, Chicken, Cow, Gerbil, Guinea pig, Pig, Xenopus laevis Click here (or use the following: https://www.abcam.com/index.html?datasheet=80779). If you require any further assistance to find a suitable nuclear marker, please do not hesitate to let me know. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Loading amount
100000 cells
Specification
mouse ES cells
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 15 2011

Application
Western blot
Loading amount
15 µg
Sample
Human Cell lysate - whole cell (MRC5VA Lung Fibroblasts)
Specification
MRC5VA Lung Fibroblasts
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 07 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Caco-2 epithelial colorectal adenocarcinoma)
Loading amount
10 µg
Specification
Human Caco-2 epithelial colorectal adenocarcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jul 07 2010

11-20 of 25 Abreviews or Q&A

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