Overview

  • Product name

    Anti-alpha Tubulin antibody
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit polyclonal to alpha Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human, Pig, Drosophila melanogaster, Zebrafish
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Xenopus laevis, Gerbil, Newt
  • Immunogen

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PEY2

  • Positive control

    • SK N SH whole cell lysate (ab3956) can be used as a positive control in WB. IHC-P: Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab15246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 17785506
IHC-Fr Use at an assay dependent concentration. PubMed: 21069788
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • This image shows a single confocal z-section of a primary porcine macrophage stained with rabbit polyclonal to alpha tubulin.

    This picture was kindly supplied as part of the review submitted by Peter Delputte (Laboratory of Virology, Ghent University, Belgium).

     

  • All lanes : Anti-alpha Tubulin antibody (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Nardi F  et al. DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130. Sci Rep 9:3035 (2019). Read more (PubMed: 30816200) »
  • Toyoda Y  et al. Functional Characterization of Clinically-Relevant Rare Variants in ABCG2 Identified in a Gout and Hyperuricemia Cohort. Cells 8:N/A (2019). Read more (PubMed: 31003562) »
See all 159 Publications for this product

Customer reviews and Q&As

1-8 of 8 Q&A

Question
Answer

We would recommend the use of one of the following products for a loading control with zebrafish samples: AB15224 anti alpha Tubulin (https://www.abcam.com/alpha-tubulin-antibody-ab15246.html). This product has been featured in the following publication for use in WB on zebrafish (PubMed ID: 22581286). AB6046 anti beta Tubulin. This product has an excellent Abreview provided by a customer using in WB on zebrafish. This review is located at the following web address: https://www.abcam.com/beta-Tubulin-antibody-Loading-Control-ab6046-reviews.html?intabreviewid=15893 AB14744 anti- COX IV antibody (https://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-Control-ab14744-references.html). Another guaranteed product for use as loading control this product has been used in publication (PubMed ID: 18765295).

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab15246 in plasmodium falciparum. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for plasmodium falciparumand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab15246.

To our knowledge, ab15246has not been tested in plasmodium falciparum. Therefore, I can offer a discount off a future purchase if you buy ab15246 now, test it in plasmodium falciparum and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab15246 in plasmodium falciparum. I will then send a discount code. This code must be issued before purchasing ab15246 so please wait for my reply before ordering.

2. Purchase ab15246either by phone, fax, or online (www.abcam.com).

3. Test it in plasmodium falciparum.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15246 turns out to be unsuitable for plasmodium falciparum, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. We would guarantee both ab15246 and ab18251 to stain cells from drosophila via immunocytochemistry (ICC/IF). I am not sure why drosophila wasn't added as a tested species to the datasheet for ab18251 after that Abreview was added, but this was an oversight and I have since updated the datasheet to include drosophila as a tested species. As for the references for ab1546, we know of them testing drosophila by WB and IHC-Fr, not ICC/IF, per se. But we would still guarantee the antibody to work in drosophila and ICC/IF since both the species and application were tested, although independently. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048163/pdf/cm0068-0001.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562427/pdf/nihms70131.pdf If you're looking for an ICC/IF protocol to use with ab1546, you can look at the below two articles, tested in mouse and human, and just apply that same protocol to your cells from drosophila. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872425/pdf/pnas.200910941.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851247/pdf/nihms120363.pdf Both of these antibodies would be covered under our Abpromise to use them with drosophila in ICC/IF so if you ran into any difficulty, we would be happy to replace or refund them for you within 6 months of your purchase. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you once again for your enquiry and for your patience. I am sorry to confirm that for Beta tubulin, the antibodies we have available tested and guaranteed in EM and mouse, rat and human tissues are mouse monoclonals. I can suggest to consider trying the following rabbit monoclonal antibody which is tested in mouse, however I am sorry this is not tested and guaranteed for EM. ab52623 Rabbit mono Anti-beta Tubulin antibody [EP1569Y] Applications Flow Cyt, ICC, IHC-P, IP, WB Reacts with: Human, Mouse, Rat Immunogen propriatory, but it is right on the C-terminus and about 15 residues long. https://www.abcam.com/index.html?datasheet=52623 https://www.abcam.com/index.html?datasheet=52623. For alpha tubulin, we have the following antibody tested in EM and mouse, rat and human but rasied in mouse. I am sorry the epitope has not been mapped: ab80779 Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free Mouse monoclonal Applications: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse, Rat, Chicken, Cow, Gerbil, Guinea pig, Pig, Xenopus laevis https://www.abcam.com/index.html?datasheet=80779 https://www.abcam.com/index.html?datasheet=80779. I understand you concerns that mouse on mouse can sometimes give non specific binding. However, this has been tested succesfully in mouse, and we are able to provide our guarantee for the results from mouse and EM. Otherwise, it could be preferable to try an antibody raised in a different species. The following rabbit polyclonal is tested in mouse, rat and human and is raised against a peptide from the C terminus. However, I am sorry it is not tested and guaranteed in EM: ab15246 Anti-alpha Tubulin antibody Rabbit polyclonal Applications: ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Pig, Rat, Zebrafish Immunogen: ALEKDYEEVGVDSVEGEGEEEGEEY C terminal amino acids 426-450 of Human alpha Tubulin https://www.abcam.com/index.html?datasheet=15246 https://www.abcam.com/index.html?datasheet=15246. With regards to the search facility on the website, I understand it is not possible to search by immunogen or region. This is partly because although we produce many antibodies in house and can provide the immunogen sequence, we do source many externally and sometimes the immunogen information is propriatory (commercially sensitive). We can usually provide this information on individual customer request. Thank you for your understanding. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your enquiry. I can confirm that the loading control protein alpha tubulin should only be located in the cytoplasm. We have several antibodies for detection of alpha tubulin which have been tested in WB, human and pig. I can recommend to review the datasheets for further information. ab15246 Anti-alpha Tubulin antibody Rabbit polyclonal Applications: ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Pig, Rat, Zebrafish Click here (or use the following: https://www.abcam.com/index.html?datasheet=15246). ab7750 Anti-alpha Tubulin antibody [TU-01] Mouse monoclonal Applications: ELISA, Flow Cyt, ICC, IF, IHC-P, IP, WB Reacts with: Human, Mouse, Arabidopsis thaliana, Cow, Dog, Pig Click here (or use the following: https://www.abcam.com/index.html?datasheet=7750). ab80779 Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free Mouse monoclonal Applications: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse, Rat, Chicken, Cow, Gerbil, Guinea pig, Pig, Xenopus laevis Click here (or use the following: https://www.abcam.com/index.html?datasheet=80779). If you require any further assistance to find a suitable nuclear marker, please do not hesitate to let me know. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your reply. As my colleague Dr Sarah Mardle has explained to you it is not our policy to provide small samples for testing. However, if an antibody does not perform as specified on the datasheet, we will provide a full refund or offer you a replacement of equivalent value. We might consider to offer a discount if you could send us a brief description of your research project. We look forward to hearing from you soon.

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Answer

Thank you for your reply. As my colleague Dr Sarah Mardle has explained to you it is not our policy to provide small samples for testing. However, if an antibody does not perform as specified on the datasheet, we will provide a full refund or offer you a replacement of equivalent value. We might consider to offer a discount if you could send us a brief description of your research project. We look forward to hearing from you soon.

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