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Dear Technical Team, Hope this e-mail finds you well! One of our customer is working on the separation of the nuclear and cytoplasmic fractions from mammalian cells like HEK 293 (HUMAN) and PS Cells(Porcine-Pig) & wants to make sure that there is no contamination of nuclear fraction in cytoplasmic fraction and vice versa. For that she wants to probe nucleus and cytoplam specific proteins in western blotting. Kindly advise the suitable antibodies which can detect the cytoplasmic proteins & not the nuclear. Looking forward towards your valued response on it. With Best Regards,
Asked on Sep 01 2011
Thank you for your enquiry. I can confirm that the loading control protein alpha tubulin should only be located in the cytoplasm. We have several antibodies for detection of alpha tubulin which have been tested in WB, human and pig. I can recommend to review the datasheets for further information. ab15246 Anti-alpha Tubulin antibody Rabbit polyclonal Applications: ICC/IF, IHC-Fr, IHC-P, IP, WB Reacts with: Fruit fly (Drosophila melanogaster), Human, Mouse, Pig, Rat, Zebrafish Click here (or use the following: https://www.abcam.com/index.html?datasheet=15246). ab7750 Anti-alpha Tubulin antibody [TU-01] Mouse monoclonal Applications: ELISA, Flow Cyt, ICC, IF, IHC-P, IP, WB Reacts with: Human, Mouse, Arabidopsis thaliana, Cow, Dog, Pig Click here (or use the following: https://www.abcam.com/index.html?datasheet=7750). ab80779 Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free Mouse monoclonal Applications: EM, Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse, Rat, Chicken, Cow, Gerbil, Guinea pig, Pig, Xenopus laevis Click here (or use the following: https://www.abcam.com/index.html?datasheet=80779). If you require any further assistance to find a suitable nuclear marker, please do not hesitate to let me know. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.
Answered on Sep 01 2011