Western blot abreview for Anti-alpha Tubulin antibody

Good
Abreviews
Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse Glioblastoma (GL261))
Specification
Mouse Glioblastoma (GL261)
Treatment
HDAC inhibitors for 24hrs
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in 1X TBS with 0.1% Tween-20
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG Antibody from Millipore
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL from WestDura (Thermo Fischer)
Exposure
30 second(s)

Additional data

Additional Notes
1. PVDF membrane (Millipore) activated with methanol for 1-2min before preparing the transfer sandwich. 2. Protease inhibitor cocktail was used. 3. For cell lysis Laemmli buffer was used. 4. Bio-Rad wet transfer was performed for 3hrs at 40volts

Abcam user community

Verified customer

Submitted Sep 12 2014

Sign up