BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in 1X TBS with 0.1% Tween-20
Non-Abcam antibody was used: Goat Anti-Rabbit IgG Antibody from Millipore Host species: Goat Clonality: Polyclonal Conjugation: Horse Radish Peroxidase
ECL from WestDura (Thermo Fischer)
1. PVDF membrane (Millipore) activated with methanol for 1-2min before preparing the transfer sandwich.
2. Protease inhibitor cocktail was used.
3. For cell lysis Laemmli buffer was used.
4. Bio-Rad wet transfer was performed for 3hrs at 40volts
Abcam user community
Submitted Sep 12 2014
Get resources and offers direct to your inboxSign up