Western blot abreview for Anti-alpha Tubulin antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA-PBS-Tween (0.1%)
Concentration
1 µg/ml

Secondary antibody

Name
Non-Abcam antibody was used: Peroxidase-IgG Fraction Monoclonal Mouse Anti-Rabb
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa

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Submitted Oct 08 2018

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