Western blot abreview for Anti-alpha Tubulin antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 21°C · Diluent: 1.5% milk
Dilution
1/2500

Secondary antibody

Dilution
1/2500
Name
Non-Abcam antibody was used: anti-Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
45 minute(s) and 0 second(s)
Bands
Specific: 55 kDa

Dr. Gilberto Betancor Quintana

Verified customer

Submitted Jun 20 2019

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