Immunohistochemistry (Frozen sections) abreview for Anti-alpha Tubulin antibody

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Tissue sections (heart)
Specification
heart
Permeabilization
Yes - 0.3% trinton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Dilution
1/2000

Additional data

Abcam response
Thanks for your honest feedback and for testing ab18251 via IHC-Fr. To our knowledge it has not been previously tested in this application.

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Submitted Sep 10 2013

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