Western blot abreview for Anti-alpha Tubulin antibody

Good
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Hela)
Specification
Hela
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS with 5% non fat milk
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Bands
Specific: 50 kDa
Exposure
30 second(s)

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Submitted Dec 12 2013

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