Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Dilution
1/8000
Incubation time
30 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
I also used this antibody for a Western Blot with a whole cell extract from HeLa and HCT116 cells at 1:10,000 and it worked extremely well. It could probably be diluted to 1:20,000 and still get very strong signal.

I have also used this antibody to immunofluorescently label Indian muntjac (IM) cells. It worked extremely well at 1:10,000. The image is an interphase IM cell labeled with anti-alpha tubulin.

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 27 2006

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