I also used this antibody for a Western Blot with a whole cell extract from HeLa and HCT116 cells at 1:10,000 and it worked extremely well. It could probably be diluted to 1:20,000 and still get very strong signal.
I have also used this antibody to immunofluorescently label Indian muntjac (IM) cells. It worked extremely well at 1:10,000. The image is an interphase IM cell labeled with anti-alpha tubulin.
Dr. Kirk Mcmanus
Submitted Feb 27 2006
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