Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha Tubulin antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER Tris/HCL pH 9.0
Sample
Cow Tissue sections (Bovine Liver)
Specification
Bovine Liver
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 30 minute(s)
Dilution
1/400

Secondary antibody

Name
Non-Abcam antibody was used: Vector ImmPRESS
Host species: Horse
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

Additional data

Additional Notes
IHC Vector Laboratories DAB Substrate for Peroxidase was used.

Dr. Steffen Rickelt

Verified customer

Submitted Aug 08 2014

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