Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDAMB231)
Permeabilization
Yes - 0.5%Triton X-100
Specification
MDAMB231
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
microtubule extraction buffer then 1% PFA

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C
Concentration
1 µg/ml

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor­ 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
MDAMB231 with H33342 (Blue) and alpha-tubulin (green) Three steps fixation, microtubule extraction buffer then 1% PFA then 1% fresh made Sodium Borohydride.

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Submitted Aug 31 2017

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