Western blot abreview for Anti-alpha Tubulin antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MC3T3)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
MC3T3
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Concentration
0.5 µg/ml

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 52 kDa

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Submitted May 10 2019

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