Overview

  • Product name

    Anti-alpha Tubulin antibody
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit polyclonal to alpha Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: Whole Zebrafish lysate; Zebrafish eye and brain lysates. IHC-Wm: Whole Zebrafish embryo.

Properties

Applications

Our Abpromise guarantee covers the use of ab233661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 50 kDa.
IHC - Wholemount 1/100 - 1/500.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • All lanes : Anti-alpha Tubulin antibody (ab233661) at 1/10000 dilution

    Lane 1 : Zebrafish brain lysate
    Lane 2 : Zebrafish eye lysate
    Lane 3 : Whole Zebrafish lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    10% SDS-PAGE

  • Zebrafish embryo tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis. 

  • Paraformaldehyde-fixed Zebrafish embryo tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis. 

  • Zebrafish embryo (2 days post-fertilization) tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis. 

  • Paraformaldehyde-fixed Zebrafish embryo (2 days post-fertilization) tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis.

References

ab233661 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233661.
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