Key features and details
- Rabbit polyclonal to alpha Tubulin
- Suitable for: WB, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-alpha Tubulin antibody
See all alpha Tubulin primary antibodies
DescriptionRabbit polyclonal to alpha Tubulin
Tested applicationsSuitable for: WB, IHC - Wholemountmore details
Species reactivityReacts with: Zebrafish
The details of the immunogen for this antibody are not available.
- WB: Whole Zebrafish lysate; Zebrafish eye and brain lysates. IHC-Wm: Whole Zebrafish embryo.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab233661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 50 kDa.|
|IHC - Wholemount||1/100 - 1/500.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
All lanes : Anti-alpha Tubulin antibody (ab233661) at 1/10000 dilution
Lane 1 : Zebrafish brain lysate
Lane 2 : Zebrafish eye lysate
Lane 3 : Whole Zebrafish lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Zebrafish embryo tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis.
Paraformaldehyde-fixed Zebrafish embryo tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis.
Zebrafish embryo (2 days post-fertilization) tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis.
Paraformaldehyde-fixed Zebrafish embryo (2 days post-fertilization) tissue stained for alpha Tubulin using ab233661 at 1/200 dilution in wholemount immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233661 has not yet been referenced specifically in any publications.