Overview

  • Product name
    Anti-alpha Tubulin antibody [B-5-1-2]
    See all alpha Tubulin primary antibodies
  • Description
    Mouse monoclonal [B-5-1-2] to alpha Tubulin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, RIA, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Chlamydomonas reinhardtii, Sea urchin
    Predicted to work with: Dog, Pig, Xenopus laevis, Cynomolgus monkey, African green monkey, Xenopus tropicalis
  • Immunogen

    Full length protein corresponding to alpha Tubulin. Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

  • Epitope
    The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.
  • Positive control
    • Total cell extract of the Human foreskin fibroblast cell line (FS11).
  • General notes

    Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 50 kDa.
RIA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF 1/100.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • All lanes : Anti-alpha Tubulin antibody [B-5-1-2] (ab11304) at 1/2000 dilution

    All lanes : Whole cell lysate prepared from HEK293 cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP conjugated mouse anti-rabbit at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds
  • ab11304 staining  alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.

  • ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

     

    See Abreview

References

This product has been referenced in:
  • Waldemer-Streyer RJ  et al. Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal. NPJ Regen Med 2:N/A (2017). Read more (PubMed: 28775895) »
  • Laker RC  et al. Ampk phosphorylation of Ulk1 is required for targeting of mitochondria to lysosomes in exercise-induced mitophagy. Nat Commun 8:548 (2017). Read more (PubMed: 28916822) »
See all 29 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary (Adult) Murine Fibroblasts)
Permeabilization
Yes - %0.3 Triton-X 100
Specification
Primary (Adult) Murine Fibroblasts
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Gokhan Yilmaz

Verified customer

Submitted Oct 06 2017

Answer

Thank you for contacting us. I can confirm that the immungen used to generate this anti-alpha Tubulin antibody clone [B-5-1-2] was a preparation of sarkosyl-resistant filaments from Stronglycentrotus purpuratus (sea urchin), as stated on the datasheet. Unfortunately, we do not have access to details about this preparation or an exact immunogen sequence for this particular antibody. However, the information we have suggests that the antibody recognises an epitope located at the C-terminal end of the alpha isoform of tubulin. Several publications cite the use of this antibody clone, unfortunately we do not know whether any of these contain information on mapping of the epitope as we do not have access to the all of the articles themselves. Preyer M et al. Interplay between Kinase Domain Autophosphorylation and F-Actin Binding Domain in Regulating Imatinib Sensitivity and Nuclear Import of BCR-ABL. PLoS One 6:e17020 (2011). PubMed: 21347248 Bou Kheir T et al. miR-449 inhibits cell proliferation and is down-regulated in gastric cancer. Mol Cancer 10:29 (2011). PubMed: 21418558 Gregersen LH et al. MicroRNA-145 targets YES and STAT1 in colon cancer cells. PLoS One 5:e8836 (2010). WB; Human. PubMed: 20098684 Sun Y et al. Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis. J Cell Biol 189:1157-69 (2010).PubMed: 20566686 Zhang C et al. Amino acid-sensing mTOR signaling is involved in modulation of lipolysis by chronic insulin treatment in adipocytes. Am J Physiol Endocrinol Metab 296:E862-8 (2009). PubMed: 19190264 Zhou J et al. mTOR supports long-term self-renewal and suppresses mesoderm and endoderm activities of human embryonic stem cells. Proc Natl Acad Sci U S A 106:7840-5 (2009). PubMed: 19416884 Barnett KR et al. The aryl hydrocarbon receptor affects mouse ovarian follicle growth via mechanisms involving estradiol regulation and responsiveness. Biol Reprod 76:1062-70 (2007). PubMed: 17329597 Gossye V et al. Differential mechanism of NF-kappaB inhibition by two glucocorticoid receptor modulators in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum 60:3241-50 (2009). PubMed: 19877072 Shelton, G.D., et al., Myopathy with tubulin-reactive inclusions in two cats. Acta Neuropathologica, 114(5), 537-542 (2007). Zhuang, Z.-G., et al., Suberoyl bis-hydroxamic acid induces p53-dependent apoptosis of MCF-7 breast cancer cells. Acta Pharmacologica Sinica, 29(12), 1459–1466 (2008). Komeno, Y., et al., AID-induced T-lymphoma or B-leukemia/lymphoma in a mouse BMT model. Leukemia, 24, 1018-1024 (2010). Banz, V.M., et al., Hsp90 transcriptionally and post-translationally regulates the expression of NDRG1 and maintains the stability of its modifying kinase GSK3β. Biochim. Biophys Acta (BBA) - Molecular Cell Research, 1793(10), 1597-1603 (2009). Gong, X., et al., RanBPM is an acetylcholinesterase-interacting protein that translocates into the nucleus during apoptosis. Acta Biochim. Biophys. Sinica, 41(11), 883-891 (2009). Okun, E., et al., TLR2 activation inhibits embryonic neural progenitor cell proliferation. J. Neurochem., 114(2), 462–474 (2010). We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Treatment
different transfection
Gel Running Conditions
Reduced Denaturing (4-12% invitrogen bis-tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2c12)
Loading amount
30 µg
Specification
C2c12
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
50 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Apr 12 2006

Answer

Thank you for your email. The best way to search the neuronal markers that we carry is to do the following: under product browser (located on the left hand side of the Abcam homepage) click on Tags & Markers, then Cell Type Markers, then Neuroscience Markers, and then Neuronal. You will then be able to view all the neuronal markers that we have. Species cross-reactivity for each antibody will be indicated on the right hand side on the list and for more specific information regarding the antibodies, please refer to the individual online datasheets. If a particular species has been tested, the information will located on the online datasheet. If you have any further questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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