Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC) (ab64503)

Overview

  • Product name
    Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (FITC)
    See all alpha Tubulin primary antibodies
  • Description
    Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Human, Pig, Xenopus laevis, Gerbil
  • Immunogen

    Full length native protein (purified) corresponding to alpha Tubulin.

  • Epitope
    aa426-450. Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin.
  • Positive control
    • ICC/IF: HeLa cells. IHC-Fr: Xenopus laevis stage 36 tissue. Flow cyt: HeLa cells. Cultured human fibroblasts, baby hamster kidney (BHK) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
Flow Cyt Use 5.1µl for 106 cells.

ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities
    Belongs to the tubulin family.
  • Post-translational
    modifications
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab64503 staining alpha Tubulin in Xenopus laevis stage 36, transverse cryosection tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with MEMFa and blocked with 2% BSA/Serum for 1 hour at 230C. The sample was incubated with primary antibody (1/100) for 1 hour at 230C. No secondary antibody was used. FITC-tubulin shown in green highlights neurons in neural tube and ciliated epidermal cells. DAPI shows staining in grey. Laminin (Abcam`s ab11575) is in blue (donkey anti-rabbit Cy5 secondary). E-cadherin is in red (donkey anti-mouse Cy3 secondary).

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:

See all 11 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (segmental nerves)
Permeabilization
Yes - triton-x
Specification
segmental nerves
Fixative
Paraformaldehyde
Username

Dr. Jacob Krans

Verified customer

Submitted Apr 01 2016

Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (segmental nerves, 3rd instar larvae, eviscerated)
Specification
segmental nerves, 3rd instar larvae, eviscerated
Username

Dr. Jacob Krans

Verified customer

Submitted Apr 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 30 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
4% formaldehyde in PHEM buffer containing 100 mM PIPES pH 6.8, 10 mM HEPES pH 7.0, 10 mM EGTA, 2mM MgCl2, 0.5-1.0% Triton X-100 for 10 minutes at room temperature
Username

Weiguo Zhang

Verified customer

Submitted Sep 08 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (stage 36, transverse cryosection, Xenopus laevis)
Specification
stage 36, transverse cryosection, Xenopus laevis
Fixative
MEMFa
Permeabilization
No
Blocking step
BSA/Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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