Western blot abreview for Anti-alpha Tubulin antibody [EP1332Y]

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Other product details

Dilution
1/50000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Odyssey blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: LI-COR N-IR 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000

Detection

Detection method
Near-infrared scan (LI-COR odyssey)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 51000 kDa

Additional data

Additional Notes
Scan was done at default sensitivity settings. Gel depicts loading controls from several animals. Samples are rat brain striatal lysate.

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Submitted Jun 17 2009

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