Application
Western blot
Loading amount
62.5 µg
Gel Running Conditions
Reduced Denaturing (12.5% acrylamide)
Sample
Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Specification
Muscle sarcoplasmic extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween
Dilution
1/40000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Pierce 31460
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL Prime
Bands
Specific: 50 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Image Key: Lane 1 and 2 - 1:20,000 dilution of primary antibody, Lane 4 and 5 - 1:40,000 dilution of primary antibody, Lane 7 and 8 - Secondary control (no primary antibody)
Dr. Shannon Cruzen
Verified customer
Submitted Feb 05 2014