Western blot abreview for Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Ovcar4 cells)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
15 µg
Specification
Ovcar4 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk / TBST
Dilution
1/10000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 50 kDa
Positive control
Lane 1- Ovcar4 Lane 2- Ovcar4 Lane 3- Ovcar4

Dr. Victoria Bridgeman

Verified customer

Submitted May 16 2016

Sign up