Western blot abreview for Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (cytoplasmic anf nuclear fraction from skin keratin)
Gel Running Conditions
Reduced Denaturing (any Kd BioRad)
Loading amount
20 µg
Specification
cytoplasmic anf nuclear fraction from skin keratin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST 0.2%
Dilution
1/5000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit HRP-linked
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
Lane 3 : Nuclear fraction
Exposure
20 second(s)
Bands
Specific: 50 kDa
Positive control
Lane 2 : cytoplasmic fraction

Additional data

Additional Notes
0.2 um nitrocellulose Lane 1 : Molecular weight markers Lane 2 : Cytoplasmic fraction Lane 3 : Nuclear fraction

Mr. Julien Coutier

Verified customer

Submitted Feb 07 2017

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