Western blot abreview for Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (osteosarcoma)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
40 µg
Treatment
Plasmid transfection using HD fuGENE
Specification
osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab (from
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 50 kDa
Positive control
alpha-tubulin is the loading control for all the samples

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Submitted Jun 27 2017

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