Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Elephant Cell (Sperm)
Permeabilization
Yes - BSA-T
Specification
Sperm
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Glutaraldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA-T
Dilution
1/250

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: AlexaFluor 680 Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680

Additional data

Additional Notes
Tried using this antibody to label tubulin in Elephant sperm. I did not see any labeling and only had labeling in the dead of the sperm using my other antibodies.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with elephant and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.

Abcam user community

Verified customer

Submitted May 31 2018

Sign up