Application
Western blot
Sample
Nematode Cell lysate - whole cell (Whole worm lysate)
Gel Running Conditions
Reduced Denaturing (4%-12% Bis-Tris)
Loading amount
5 µg
Specification
Whole worm lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
19 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye® 800CW anti-Rabbit IgG #926-32213
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odissey scan
Exposure
1 second(s)
Bands
Specific: 50 kDa
Positive control
U2OS cell lysate (5µg) shows the same 50kDa specific band with similar intensity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Sonia Ravanelli
Verified customer
Submitted Nov 30 2020