Key features and details
- Rabbit polyclonal to alpha Tubulin (HRP)
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Product nameAnti-alpha Tubulin antibody (HRP)
See all alpha Tubulin primary antibodies
DescriptionRabbit polyclonal to alpha Tubulin (HRP)
SpecificityCross reactivity will occur with all isoforms of alpha tubulin.
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
General notesDo NOT add Sodium Azide!
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
BSA is Immunoglobulin and Protease free
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab106043 was affinity purified from monospecific antiserum by immunoaffinity purification.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
ab106043 has been referenced in 1 publication.
- Yang YH et al. Stromal Tissue Rigidity Promotes Mesenchymal Stem Cell-Mediated Corneal Wound Healing Through the Transforming Growth Factor ß Signaling Pathway. Stem Cells 34:2525-2535 (2016). PubMed: 27250866