Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody - Loading Control

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - PBST for 30 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
4% formaldehyde in PBS/0.2% Triton X-100

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti- chicken Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
For primary antibodies, chicken anti- alpha-tubulin 1:400 (green), rabbit anti- CENP-B 1:200 (Abcam ab25734) (blue), mouse anti- HEC1 1:400 (Abcam ab3613) (red). Secondary antibodies were goat anti- chicken Alexa488, goat anti- rabbit Alexa546, goat anti- mouse Alexa647 used at 1:400 for 1 hr at room temperature. The three antibodies allow simultaneous visualization of spindle microtubules, inner centromere and outer kinetochore structures as well as DNA stained by DAPI.

Weiguo Zhang

Verified customer

Submitted Aug 25 2015

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