For primary antibodies, chicken anti- alpha-tubulin 1:400 (green), rabbit anti- CENP-B 1:200 (Abcam ab25734) (blue), mouse anti- HEC1 1:400 (Abcam ab3613) (red). Secondary antibodies were goat anti- chicken Alexa488, goat anti- rabbit Alexa546, goat anti- mouse Alexa647 used at 1:400 for 1 hr at room temperature. The three antibodies allow simultaneous visualization of spindle microtubules, inner centromere and outer kinetochore structures as well as DNA stained by DAPI.
Submitted Aug 25 2015
Get resources and offers direct to your inboxSign up